The doi above is for this specific version of this dataset, which is currently the latest. Newer versions may be published in the future. For a link that will always point to the latest version, please use
doi: 10.4121/ddc72065-1082-4183-908d-a55b04d05cb2

Tschirpke, Sophie; Laan, Liedewij; van Opstal, Frank; van der Valk, Ramon; Daalman, Werner (2023): Data underlying the publication: A guide to the in vitro reconstitution of Cdc42 GTPase activity and its regulation. Version 1. 4TU.ResearchData. dataset. https://doi.org/10.4121/ddc72065-1082-4183-908d-a55b04d05cb2.v1

Dataset

• Biochemistry and Cell Biology
• Biological Sciences

Cdc42, Cdc42-Cdc24, cell division, eukaryotes, GEF, gtpase activity, in vitro

CC BY-NC 4.0

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by Sophie Tschirpke , Liedewij Laan , Frank van Opstal , Ramon van der Valk , Werner Daalman

We investigated the in vitro GTPase activity of the S.cerevisae GTPase Cdc42, alone and in combination with its GDP/GTP exchange factor (GEF) Cdc24. We used the GTPase assay 'GTPase-Glo assay' by Promega. The data consists of assay data (amount of remaining GTP for various time points and protein concentrations) for variously tagged Cdc42 constructs and purification batches.

• 2023-07-17 first online, published, posted

4TU.ResearchData

xlsx

A guide to the in vitro reconstitution of Cdc42 GTPase activity and its regulation

• funding from the European Research Council under the European Union’s Horizon 2020 research and innovation programme (grant agreement 758132) (grant code 758132) European Research Council
• funding from the Netherlands Organization for Scientific Research (Nederlandse Organisatie voor Wetenschappelijk Onderzoek) through a Vidi grant (016.Vidi.171.060) (grant code 016.Vidi.171.060) Netherlands Organization for Scientific Research

TU Delft, Faculty of Applied Sciences, Department of Bionanoscience
TU Delft, Kavli Institute of Nanoscience