cff-version: 1.2.0
abstract: "<p>We investigated the<em> in vitro </em>GTPase activity of the <em>S.cerevisae</em> GTPase Cdc42, alone and in combination with its GDP/GTP exchange factor (GEF) Cdc24. We used the GTPase assay 'GTPase-Glo assay' by Promega. The data consists of assay data (amount of remaining GTP for various time points and protein concentrations) for variously tagged Cdc42 constructs and purification batches.</p>"
authors:
  - family-names: Tschirpke
    given-names: Sophie
    orcid: "https://orcid.org/0009-0005-6550-9291"
  - family-names: Laan
    given-names: Liedewij
    orcid: "https://orcid.org/0000-0002-7138-9004"
  - family-names: van Opstal
    given-names: Frank
  - family-names: van der Valk
    given-names: Ramon
  - family-names: Daalman
    given-names: Werner
title: "Data underlying the publication: A guide to the in vitro reconstitution of Cdc42 GTPase activity and its regulation"
keywords:
version: 1
identifiers:
  - type: doi
    value: 10.4121/ddc72065-1082-4183-908d-a55b04d05cb2.v1
license: CC BY-NC 4.0
date-released: 2023-07-17