TY - DATA
T1 - Data underlying the publication: A guide to the in vitro reconstitution of Cdc42 GTPase activity and its regulation
PY - 2023/07/17
AU - Sophie Tschirpke
AU - Liedewij Laan
AU - Frank van Opstal
AU - Ramon van der Valk
AU - Werner Daalman
UR - 
DO - 10.4121/ddc72065-1082-4183-908d-a55b04d05cb2.v1
KW - Cdc42
KW - gtpase activity
KW - Cdc42-Cdc24
KW - GEF
KW - cell division
KW - eukaryotes
KW - in vitro
N2 - <p>We investigated the<em> in vitro </em>GTPase activity of the <em>S.cerevisae</em> GTPase Cdc42, alone and in combination with its GDP/GTP exchange factor (GEF) Cdc24. We used the GTPase assay 'GTPase-Glo assay' by Promega. The data consists of assay data (amount of remaining GTP for various time points and protein concentrations) for variously tagged Cdc42 constructs and purification batches.</p>
ER -