Population growth rate data and scripts concerning the publication:"Redundancy and the role of protein copy numbers in the cell polarization machinery of budding yeast"experiments
doi: 10.4121/67e56fe8-6b54-446b-aaac-cd2e245ee066
CC BY-NC 4.0
The data entails raw and processed data from population growth rates experiments using different yeast mutants. Moreover, it contains the required scripts to process
the data and reproduce Fig3 and Supplemental Figures 4 and 5 from the paper "Redundancy and the role of protein copy numbers in the cell polarization machinery of budding yeast" published in Nature Communications.
The experiments investigated the role of Cdc42 copy number in the polarization efficiency of different relevant mutants containing knockout mutations in Bem1 and Bem3 genes. The Cdc42 copy number was controlled using a galactose promoter.
Initially the growth measurements were taken using a sfGFP tag fussed in Cdc42 (data shown in Fig3). Therefore, we did control experiments, shown in the Supplemental material, to check that the sfGFP tag was not influencing the results we observed previously (Supp Fig 4 and Supp Fig 5).
- 2023-07-19 first online, published, posted
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