Data underlying the publication: Image-based phenotypic sorting of synthetic cells

DOI:10.4121/60cdce27-5bd5-44ec-9d8f-53f2ef6b9d58.v1
The DOI displayed above is for this specific version of this dataset, which is currently the latest. Newer versions may be published in the future. For a link that will always point to the latest version, please use
DOI: 10.4121/60cdce27-5bd5-44ec-9d8f-53f2ef6b9d58

Datacite citation style

van den Brink, Marijn; Stam, Marlena; Claassens, Nico; Danelon, Christophe (2025): Data underlying the publication: Image-based phenotypic sorting of synthetic cells. Version 1. 4TU.ResearchData. dataset. https://doi.org/10.4121/60cdce27-5bd5-44ec-9d8f-53f2ef6b9d58.v1
Other citation styles (APA, Harvard, MLA, Vancouver, Chicago, IEEE) available at Datacite

Dataset

Data underlying the publication: Van den Brink, M., Stam, M., Claassens, N.J. and Danelon, C. (2025) Image-based phenotypic sorting of synthetic cells. bioRxiv, 10.1101/2025.09.25.678515.


This dataset contains data collected during experiments as part of Marijn van den Brink's PhD project. The data was collected from 2022-2025.


The raw data consist of microscopy data, gel electrophoresis data, flow cytometry data, quantitative PCR data, DNA sequencing data. All data processing and analysis steps are described in detail in the Methods section of the publication.


The data are grouped in folders based on three levels. Primary, by the data type. Secondary, by the figure number in the publication. Tertiary, within each figure folder, data are divided into raw and processed subfolders. The publication figures are stored in a separate folder.


Large microscopy data collections (e.g., > 2 GB), generated during automated screening and photoactivation, are stored in separate folders, so they can be downloaded separately.


Code or other files used to process the raw data are stored in the "processed data" folders.


Generalized code for use and adaptation by others is stored in the folder "automated screening and photo activation scripts" and are also published on GitHub (https://github.com/DanelonLab).

History

  • 2025-09-26 first online, published, posted

Publisher

4TU.ResearchData

Format

spreadsheet/.xlsx; spreadsheet and figures/.pzfx; image/.nd2; image/.jpg; image/.tif; image/.scn; Nanopore sequencing reads/.fastq; aligned sequences/BAM; FACS data/.fcs; FACS data/.txt; qPCR data/.eds; DNA sequences/.dna; script/.ipynb; script/.py; script/.R; script/.bat; Nis-Elements JOB/.bin

Associated peer-reviewed publication

Image-based phenotypic sorting of synthetic cells

Funding

  • BaSyC – Building a Synthetic Cell Gravitation grant (grant code 024.003.019) NWO
  • ANR grant (grant code ANR-22-CPJ2-0091-01) Agence Nationale de la Recherche
  • Evolving life from non-life (EVOLF) (grant code SUMMIT.1.004) NWO

Organizations

TU Delft, Faculty of Applied Sciences, Department of Bionanoscience

DATA

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