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Data underlying the research on Identification of oxygen-independent pathways for pyridine-nucleotide and Coenzyme-A synthesis in anaerobic fungi by expression of candidate genes in yeast.

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posted on 02.07.2020 by Jean-Marc Daran, T. (Thomas) Perli, Vos A.M. (Aurin), Ortiz-Merino R.A. (Raúl), Pronk J.T. (Jack)
Neocallimastigomycetes are rare examples of strictly anaerobic eukaryotes. This study investigates how these anaerobic fungi bypass reactions involved in synthesis of pyridine nucleotide cofactors and coenzyme A that in canonical fungal pathways require molecular oxygen. Analysis of Neocallimastigomycete proteomes identified a candidate L-aspartate-decarboxylase (panD) and a bifunctional L-aspartate oxidase/quinolinate synthase (nadB/nadA) . The corresponding gene sequences indicated early ancient acquisition by horizontal gene transfer from bacterial donors. To test whether these enzymes suffice to bypass corresponding oxygen-requiring reactions, they were introduced into fms1Δ and bna2Δ Sacharomyces cerevisiae strains. Expression of nadA/, nadB and panD from the Neocallimastigomycetes Piromyces finnis and Neocallimastix californiae, respectively, conferred cofactor prototrophy under aerobic and anaerobic conditions. This study simulates how horizontal gene transfer can drive eukaryotic adaptation to anaerobiosis, and provides a basis for elimination of auxotrophic requirements in anaerobic industrial applications of yeasts and fungi.


European Research Council, Eloxy 694633

European Union’s Horizon 2020 research and innovation programme, Pacmen 722287



Bouwknegt J. (Jonna) [orcid:0000-0316-1238-7873]; Dekker W.J.C. (Wijb); TU Delft, Faculty of Applied Sciences, Department of Biotechnology; Wiersma S.J. (Sanne)


4TU.Centre for Research Data


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