The doi above is for this specific version of this dataset, which is currently the latest. Newer versions may be published in the future. For a link that will always point to the latest version, please use
doi: 10.4121/21494520

Krzysica, Paulina; Verhoog, Loes; de Vries, Sonja; Smits, Coen; Savelkoul, H.F.J. (Huub) et. al. (2022): Dataset underlying the publication 'Optimization of Capture ELISAs for Chicken Cytokines Using Commercially Available Antibodies''. Version 1. 4TU.ResearchData. dataset. https://doi.org/10.4121/21494520.v1

Dataset

• Other Agricultural and Veterinary Sciences
• Veterinary Sciences
• Immunology

antibodies, capture ELISA, chicken, cytokines, interferon, interleukin, poultry

2018-2019

CC BY 4.0

RefWorks, BibTeX, Reference Manager, Endnote, DataCite, NLM, DC, CFF

by Paulina Krzysica, Loes Verhoog , Sonja de Vries , Coen Smits, H.F.J. (Huub) Savelkoul , E.J. (Edwin) Tijhaar

The research is about optimization and validation of capture ELISA for chicken IL-2, IL-6, IL-10, IL-12p40, and IFN-y using commercially available antibodies and recombinant cytokines.

To optimize antibody concentrations, checkerboard titration was performed. Then, validation tests for capture ELISAs were performed to assess lower limit of detection (LLOD), matrix effect, parallelism, and inter-assay variation.

• 2022-12-09 first online, published, posted

4TU.ResearchData

xlsx

Optimization of Capture ELISAs for Chicken Cytokines Using Commercially Available Antibodies

Cell Biology and Immunology, Wageningen University & Research
Animal Nutrition, Wageningen University & Research