cff-version: 1.2.0
abstract: "<p>The research is about optimization and validation of capture ELISA for chicken IL-2, IL-6, IL-10, IL-12p40, and IFN-y using commercially available antibodies and recombinant cytokines.</p>
<p>To optimize antibody concentrations, checkerboard titration was performed. Then, validation tests for capture ELISAs were performed to assess lower limit of detection (LLOD), matrix effect, parallelism, and inter-assay variation.</p>"
authors:
  - family-names: Krzysica
    given-names: Paulina
  - family-names: Verhoog
    given-names: Loes
    orcid: "https://orcid.org/0000-0003-1621-7362"
  - family-names: de Vries
    given-names: Sonja
    orcid: "https://orcid.org/0000-0002-3842-8411"
  - family-names: Smits
    given-names: Coen
  - family-names: Savelkoul
    given-names: H.F.J. (Huub)
    orcid: "https://orcid.org/0000-0001-6236-7092"
  - family-names: Tijhaar
    given-names: E.J. (Edwin)
    orcid: "https://orcid.org/0000-0002-9014-0407"
title: "Dataset underlying the publication &#39;Optimization of Capture ELISAs for Chicken Cytokines Using Commercially Available Antibodies&#39;&#39;"
keywords:
version: 1
identifiers:
  - type: doi
    value: 10.4121/21494520.v1
license: CC BY 4.0
date-released: 2022-12-09