TY - DATA
T1 - Dataset underlying the publication 'Optimization of Capture ELISAs for Chicken Cytokines Using Commercially Available Antibodies''
PY - 2022/12/09
AU - Paulina Krzysica
AU - Loes Verhoog
AU - Sonja de Vries
AU - Coen Smits
AU - H.F.J. (Huub) Savelkoul
AU - E.J. (Edwin) Tijhaar
UR - https://data.4tu.nl/articles/dataset/Dataset_underlying_the_publication_Optimization_of_Capture_ELISAs_for_Chicken_Cytokines_Using_Commercially_Available_Antibodies_/21494520/1
DO - 10.4121/21494520.v1
KW - chicken
KW - poultry
KW - capture ELISA
KW - antibodies
KW - cytokines
KW - interleukin
KW - interferon
N2 - <p>The research is about optimization and validation of capture ELISA for chicken IL-2, IL-6, IL-10, IL-12p40, and IFN-y using commercially available antibodies and recombinant cytokines.</p>
<p>To optimize antibody concentrations, checkerboard titration was performed. Then, validation tests for capture ELISAs were performed to assess lower limit of detection (LLOD), matrix effect, parallelism, and inter-assay variation.</p>
ER -