%0 Generic
%A Krzysica, Paulina
%A Verhoog, Loes
%A de Vries, Sonja
%A Smits, Coen
%A Savelkoul, H.F.J. (Huub)
%A Tijhaar, E.J. (Edwin)
%D 2022
%T Dataset underlying the publication 'Optimization of Capture ELISAs for Chicken Cytokines Using Commercially Available Antibodies''
%U https://data.4tu.nl/articles/dataset/Dataset_underlying_the_publication_Optimization_of_Capture_ELISAs_for_Chicken_Cytokines_Using_Commercially_Available_Antibodies_/21494520/1
%R 10.4121/21494520.v1
%K chicken
%K poultry
%K capture ELISA
%K antibodies
%K cytokines
%K interleukin
%K interferon
%X <p>The research is about optimization and validation of capture ELISA for chicken IL-2, IL-6, IL-10, IL-12p40, and IFN-y using commercially available antibodies and recombinant cytokines.</p>
<p>To optimize antibody concentrations, checkerboard titration was performed. Then, validation tests for capture ELISAs were performed to assess lower limit of detection (LLOD), matrix effect, parallelism, and inter-assay variation.</p>
%I 4TU.ResearchData