Data underlying the publication: Oligomerization-dependent and synergistic regulation of Cdc42 GTPase cycling by a GEF and a GAP

DOI:10.4121/56535d83-8a7a-4367-9ae5-f0d56b51161f.v1
The DOI displayed above is for this specific version of this dataset, which is currently the latest. Newer versions may be published in the future. For a link that will always point to the latest version, please use
DOI: 10.4121/56535d83-8a7a-4367-9ae5-f0d56b51161f

Datacite citation style

Tschirpke, Sophie; Daalman, Werner; Laan, Liedewij (2025): Data underlying the publication: Oligomerization-dependent and synergistic regulation of Cdc42 GTPase cycling by a GEF and a GAP. Version 1. 4TU.ResearchData. dataset. https://doi.org/10.4121/56535d83-8a7a-4367-9ae5-f0d56b51161f.v1
Other citation styles (APA, Harvard, MLA, Vancouver, Chicago, IEEE) available at Datacite

Dataset

This dataset contains data collected for the publication 'Oligomerization-dependent and synergistic regulation of Cdc42 GTPase cycling by a GEF and a GAP' (doi: https://doi.org/10.1101/2023.06.26.546500). The data contains GTPase Glo assay data and SEC-MALS data.

GTPase assay data: The purpose of these experiments was to quantify the GTPase activity of the S.cerevisae GTPase Cdc42, alone and in combination with its GEF Cdc24 and GAP Rga2, and variants thereof (Cdc24-DH3, GAP domain). We used the GTPase assay 'GTPase-Glo assay' by Promega. The data consists of assay data where different concentrations of the mentioned proteins, in different combinations, are used.

(2) SEC-MALS data: The purpose was to asses the oligomeric state of S.cerevisae proteins Cdc24, Rga2, and the GAP domain of Rga2.

History

  • 2025-06-12 first online, published, posted

Publisher

4TU.ResearchData

Format

.txt, .xlsx, .ipynb

Funding

  • Netherlands Organization for Scientific Research (Nederlandse Organisatie voor Wetenschappelijk Onderzoek) through a Vidi grant (016.Vidi.171.060) (grant code 016.Vidi.171.060) Nederlandse Organisatie voor Wetenschappelijk Onderzoek
  • Kavli Synergy Post-doctoral Fellowship program of the Kavli Institute of Nanoscience Delft Kavli Institute of Nanoscience Delft

Organizations

TU Delft, Faculty of Applied Sciences, Department of Bionanoscience
TU Delft, Kavli Institute of Nanoscience

DATA

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