TY - DATA
T1 - Data underlying the publication: Oligomerization-dependent and synergistic regulation of Cdc42 GTPase cycling by a GEF and a GAP
PY - 2025/06/12
AU - Sophie Tschirpke
AU - Werner Daalman
AU - Liedewij Laan
UR - 
DO - 10.4121/56535d83-8a7a-4367-9ae5-f0d56b51161f.v1
KW - GTPase assay
KW - SEC-MALS
KW - protein-protein interaction
KW - protein kinetics
KW - protein oligomerization
N2 - <p>This dataset contains data collected for the publication 'Oligomerization-dependent and synergistic regulation of Cdc42 GTPase cycling by a GEF and a GAP' (doi: https://doi.org/10.1101/2023.06.26.546500). The data contains GTPase Glo assay data and SEC-MALS data. </p><p>GTPase assay data: The purpose of these experiments was to quantify the GTPase activity of the S.cerevisae GTPase Cdc42, alone and in combination with its GEF Cdc24 and GAP Rga2, and variants thereof (Cdc24-DH3, GAP domain). We used the GTPase assay 'GTPase-Glo assay' by Promega. The data consists of assay data where different concentrations of the mentioned proteins, in different combinations, are used. </p><p>(2) SEC-MALS data: The purpose was to asses the oligomeric state of S.cerevisae proteins Cdc24, Rga2, and the GAP domain of Rga2.</p>
ER -