Analyzing Candida albicans enolase with MAbs

Datacite citation style:
Zhengxin He (2021): Analyzing Candida albicans enolase with MAbs. Version 1. 4TU.ResearchData. dataset. https://doi.org/10.4121/15040701.v1
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Here we investigate these features in extract peptone dextrose (YPD) medium using a pair of monoclonal antibodies which were validated by Western blot, and LC MS/MS.
CaEno abundantly presented in the cytosol distributing in a ring-shaped pattern along the cell wall;
Surface display of CaEno was confirmed by whole cell ELISA and flow cytometry. Further laser confocal microscopy analysis revealed that the presence of the surface-located CaEno maintained stable during the culture.
DAS-ELISA indicated that CaEno in the supernatant was gradually increased during culture and reached a maximum at 48 h and then the concentration remained stable until 120 h.
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  • 2021-08-10 first online, published, posted
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4TU.ResearchData
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xlsx
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Bethune International Peace Hospital, Basic Medical laboratory, Shijiazhuang, Hebei

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