TY - DATA
T1 - Analyzing Candida albicans enolase with MAbs
PY - 2021/08/10
AU - Zhengxin He
UR - https://data.4tu.nl/articles/dataset/Analyzing_Candida_albicans_enolase_with_MAbs/15040701/1
DO - 10.4121/15040701.v1
KW - Enolase
KW - candida albicans
KW - monoclonal antibody
KW - subcellular location
KW - growth
N2 - Here we investigate these features in extract peptone
dextrose (YPD) medium using a pair
of monoclonal antibodies which were validated by Western blot, and LC MS/MS. <div>CaEno abundantly
presented in the cytosol distributing in a ring-shaped pattern along the cell
wall;</div><div>Surface display of CaEno was confirmed by whole cell ELISA and flow cytometry. Further laser confocal
microscopy analysis revealed that the presence of the surface-located CaEno maintained stable
during the culture.</div><div>DAS-ELISA indicated that CaEno in the supernatant was
gradually increased during culture and reached a maximum at 48 h and then the
concentration remained stable until 120 h. </div>
ER -