Data underlying publication: Spinach Seed Microbiome Characteristics Linked to Suppressiveness Against Globisporangium ultimum Damping-Off

DOI:10.4121/03e9e2af-904e-4949-ac10-c5721fd6e0f1.v5
The DOI displayed above is for this specific version of this dataset, which is currently the latest. Newer versions may be published in the future. For a link that will always point to the latest version, please use
DOI: 10.4121/03e9e2af-904e-4949-ac10-c5721fd6e0f1

Datacite citation style

Makrina Diakaki; Andreo Jimenez, Beatriz; de Lange, Ezra; Patrick Butterbach; Liesbeth van der Heijden et. al. (2025): Data underlying publication: Spinach Seed Microbiome Characteristics Linked to Suppressiveness Against Globisporangium ultimum Damping-Off. Version 5. 4TU.ResearchData. dataset. https://doi.org/10.4121/03e9e2af-904e-4949-ac10-c5721fd6e0f1.v5
Other citation styles (APA, Harvard, MLA, Vancouver, Chicago, IEEE) available at Datacite

Recently we demonstrated that the seed microbiome of certain spinach (Spinacia oleracea) seed lots can confer disease suppression against Globisporangium ultimum damping-off (previously known as Pythium ultimum). We hypothesised that differences in the microbial community composition of spinach seed lots correlate with the levels of damping-off suppressiveness of each seed lot. Here, we show that a large proportion of variance in seed-associated bacterial (16S) and fungal (ITS1) amplicon sequences was explained by seed lot identity, while 9.8 % of bacterial and 7.1 % of fungal community variance correlated with disease suppression. More specifically, a higher relative abundance of basidiomycetous dimorphic yeasts such as Vishniacozyma, Filobasidium and Papiliotrema and of the bacterial genus Massilia was a key feature of suppressive seed microbiomes. We suggest that the abundance of these genera is indicative of seed lot suppressive potential. Seed processing and treatment can become more targeted with indicator taxa being used to evaluate the presence of beneficial seed-associated microbial functions. This process in turn could contribute to the sustainable management of seedling diseases. Finally, this study highlights the ubiquity of yeasts in spinach seed microbiota and their potential beneficial roles for seed health.


History

  • 2024-09-13 first online
  • 2025-01-14 published, posted

Publisher

4TU.ResearchData

Format

script/.R, spreadsheet/.csv OR .txt, Qiime output/.qza, word files/.docx OR .txt, zipped files/ .zip

Funding

  • LWV19097; Top Sector Horticulture and Propagation Materials

Organizations

Wageningen Plant Research, Wageningen University and Research
Soil Biology Group, Wageningen University and Research
Bejo Zaden B.V., Warmenhuizen, The Netherlands
Germains Seed Technology, Enkhuizen, The Netherlands
Department of Microbial Ecology, Netherlands Institute of Ecology (NIOO-KNAW)

DATA

Files (24)