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Data underlying the manuscript: “Modular, synthetic chromosomes as new tools for large scale engineering of metabolism”

posted on 22.11.2021, 16:26 authored by Jean-Marc DaranJean-Marc Daran, Else-Jasmijn Hassing

These data related to the manuscript Modular, synthetic chromosomes as new tools for large scale engineering of metabolism” included as chapter 4 of Else-Jasmijn Hassing's PhD thesis entitled “Metabolic engineering of Saccharomyces cerevisiae for the production of aromatic compounds”.

The construction of powerful cell factories requires intensive genetic engineering for the addition of new functionalities and the remodeling of native pathways and processes. The present study, using Saccharomyces cerevisiae, explores the potential of modular, specialized de novo-assembled neochromosomes (named NeoChrs) to facilitate extensive genome reprogramming. Linear and circular NeoChrs carrying 20 native and 21 heterologous genes were designed and constructed by in vivo assembly in yeast in two transformation steps, leading to de novo production of anthocyanins, native to plants. Turned into exclusive expression platforms for heterologous and essential metabolic routes, the NeoChrs behaved like native chromosomes regarding mitotic and genetic stability, copy number, harmlessness for the host and editability by CRISPR-Cas. This study paves the way for future microbial cell factories with modular genomes in which core metabolic networks, localized on satellite, specialized NeoChrs can be swapped for alternative configurations and serve as landing pads for addition of functionalities.


Model-Based Construction And Optimisation Of Versatile Chassis Yeast Strains For Production Of Valuable Lipid And Aromatic Compounds

European Commission

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Delft University of Technology, Applied Sciences, Department of Biotechnology