Analyzing Candida albicans enolase with MAbs

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Zhengxin He (2021): Analyzing Candida albicans enolase with MAbs. Version 1. 4TU.ResearchData. dataset.
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choose version: version 3 - 2022-04-19 (latest) version 2 - 2021-08-11
version 1 - 2021-08-10
Here we investigate these features in extract peptone dextrose (YPD) medium using a pair of monoclonal antibodies which were validated by Western blot, and LC MS/MS.
CaEno abundantly presented in the cytosol distributing in a ring-shaped pattern along the cell wall;
Surface display of CaEno was confirmed by whole cell ELISA and flow cytometry. Further laser confocal microscopy analysis revealed that the presence of the surface-located CaEno maintained stable during the culture.
DAS-ELISA indicated that CaEno in the supernatant was gradually increased during culture and reached a maximum at 48 h and then the concentration remained stable until 120 h.
  • 2021-08-10 first online, published, posted
Bethune International Peace Hospital, Basic Medical laboratory, Shijiazhuang, Hebei


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