Analyzing Candida albicans enolase with MAbs
Datacite citation style:
Zhengxin He (2021): Analyzing Candida albicans enolase with MAbs. Version 1. 4TU.ResearchData. dataset. https://doi.org/10.4121/15040701.v1Other citation styles (APA, Harvard, MLA, Vancouver, Chicago, IEEE) available at Datacite
Here we investigate these features in extract peptone dextrose (YPD) medium using a pair of monoclonal antibodies which were validated by Western blot, and LC MS/MS.
CaEno abundantly presented in the cytosol distributing in a ring-shaped pattern along the cell wall;
Surface display of CaEno was confirmed by whole cell ELISA and flow cytometry. Further laser confocal microscopy analysis revealed that the presence of the surface-located CaEno maintained stable during the culture.
DAS-ELISA indicated that CaEno in the supernatant was gradually increased during culture and reached a maximum at 48 h and then the concentration remained stable until 120 h.
- 2021-08-10 first online, published, posted
organizationsBethune International Peace Hospital, Basic Medical laboratory, Shijiazhuang, Hebei
f6cca20f53a0bcd6fcdf37aa74ed09b210H8 LC MS identification.xlsx
549a297ae412ea0b924e146cd5126bf79H8 LC MS identification.xlsx
da5e9ef964da98c50c4116c87c64d812confocal AD values.xlsx
d5d9a26314fe104bc3956cf8f1902724DAS-ELISA standard curve.xlsx
08124933dbe14eedb5c20170e72c6244Enolase in supernatant.xlsx
19101a6ca748811e5d29eff8f658e6d7growth curve of C. albicans culture in YPD.xlsx
d254764a657a2ef92d08f31b25228979whole cell ELISA.xls
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