%0 Generic
%A He, Zhengxin
%D 2021
%T Analyzing Candida albicans enolase with MAbs
%U https://data.4tu.nl/articles/dataset/Analyzing_Candida_albicans_enolase_with_MAbs/15040701/1
%R 10.4121/15040701.v1
%K Enolase
%K candida albicans
%K monoclonal antibody
%K subcellular location
%K growth
%X Here we investigate these features in extract peptone
dextrose (YPD) medium using a pair
of monoclonal antibodies which were validated by Western blot, and LC MS/MS. <div>CaEno abundantly
presented in the cytosol distributing in a ring-shaped pattern along the cell
wall;</div><div>Surface display of CaEno was confirmed by whole cell ELISA and flow cytometry. Further laser confocal
microscopy analysis revealed that the presence of the surface-located CaEno maintained stable
during the culture.</div><div>DAS-ELISA indicated that CaEno in the supernatant was
gradually increased during culture and reached a maximum at 48 h and then the
concentration remained stable until 120 h. </div>
%I 4TU.ResearchData