Data underlying the publication: Clonal Amplification-Enhanced Gene Expression in Synthetic Vesicles
doi: 10.4121/f9d102ba-bf4d-4dae-b6ed-88985747ea2a
Data behind the publication: 1. Abil, Z., Restrepo Sierra, A. M. & Danelon, C. Clonal Amplification-Enhanced Gene Expression in Synthetic Vesicles. ACS Synth. Biol. 12, 1187–1203 (2023).
The hereby provided research was designed and performed as part of the synthetic biology and BaSyc (NL) consortium efforts to build a synthetic cell from the bottom up. The dataset includes '.prism' files with the raw data utilized for constructing the figures from the above stated paper. Besides these files, the provided .zip files also contain raw data that includes both flow cytometry and confocal microscopy data. Each compressed file indicates to which figure(s) it should belong to and which type of data does it contain. Some folders (i.e., some of the ones with FACS data) also include a .MATLAB file with a small script connected to each file for easier visualization of the data. The confocal imaging data (if not in PNG) can be opened and analyzed with FIJI. The dataset also includes two separate MATLAB files labeled with 'SMELDit', two MATLAB scripts utilized for the analysis of confocal images from Fig. 6 of the above mentioned paper. The additional PDF labeled: MEP_thesis_Mats van Tongeren contains a more specific description and instructions on how to operate the program. Finally, the dataset also contains the SnapGene files with the different DNA sequences utilized on the experiments of the above mentioned paper.
If additional information is needed, please contact a.m.restreposierra@tudelft.nl or anasierra.93@gmail.com, or also the corresponding author of the paper: Christophe Danelon.
- 2023-12-08 first online, published, posted
DATA
- 3,265 bytesMD5:
fa620df445fb408c4e2d4e3aff2a50cc
README.txt - 641,924,676 bytesMD5:
834fcac02839db6bb8d8a92cd21c8a93
2020-07-02_confocal_oriminD_Fig6.zip - 1,716,179,779 bytesMD5:
e7c06410e29be0107234bf22a1bf23c8
2020-07-07_confocal_oriminD_Fig6.zip - 11,123,016 bytesMD5:
554b249a649cada332e30d2c5e171275
clonal-amplification-enhanced-gene-expression-in-synthetic-vesicles.pdf - 311,748,809 bytesMD5:
2c8917edc174ad63718fe8ffe4768f24
Confocal_2020-07-23_confocal_oriYFP_Fig2.zip - 1,899,654 bytesMD5:
ac5cd0dbb1b5217a26d31630cbbb0d83
Confocal_CADGE oriPSSA_Fig5.zip - 145,556,983 bytesMD5:
193246e933be389783ec68276cec9746
Confocal_Titration oriPSSA_FigS7.zip - 8,906,598 bytesMD5:
13e7f809d3da9158aa89c1d3be5a1d20
FACS_oriYFP exp proteins 23-06-2020_spill_applied_events_Fig 2.zip - 3,600,306 bytesMD5:
bf6f6504b5b19e2f636edaf693bae815
FACS_oriYFP exp proteins 23-07-2020_spill_applied_events_Fig 2.zip - 7,809,354 bytesMD5:
6da44dfc58ed0ddafa25fee723d4f5db
FACS_oriYFP purified proteins 27-10-2020_spill_applied_events_Fig 2.zip - 40,042,986 bytesMD5:
6d1043bd9e6f332f4aeb8434f2fde659
FACS_Replicate #1 oriYFPoriMinD Fig4.zip - 25,248,778 bytesMD5:
d56adaa9c079f1e0ef522225d7ec929c
FACS_Replicate #2 oriYFPoriMinD Fig4.zip - 20,125,429 bytesMD5:
806d9338f79df9c609177a21059641d9
FACS_Replicate #3 oriYFPoriMinD Fig4.zip - 19,120,359 bytesMD5:
26a2f93b2b139d2659050feed81afe1c
FACS_Replicate #4 oriYFPoriMinD Fig4.zip - 176,100 bytesMD5:
2d250cbaf3edbad3f3f5af14349c3be9
Figure1.prism - 93,184 bytesMD5:
b57ec5cd69ac7ca37b9f0bd7ddc88be2
Figure2_1.prism - 23,736 bytesMD5:
71ed6ff76f54dd9b849779f70a0eaa8b
Figure2_2.prism - 45,868 bytesMD5:
4b5d09ec6c32be5aa8027ef969703daa
Figure3.prism - 61,229 bytesMD5:
7d438b7826b90cef5d6b4cbe6874379b
Figure4.prism - 49,043 bytesMD5:
944678e2d15ff9b59714edc0e3f5e8f2
Figure5.prism - 22,423 bytesMD5:
babdf15d07e0e2ed7530ef7d87c72b21
Figure6.prism - 15,718 bytesMD5:
424f0da519a75a24411236e4221a23db
Load_Recognized_liposomes_SMELDit MATLAB.m - 5,956,561 bytesMD5:
4276251316317397b5cbe5a62e99d8d8
MEP_thesis_Mats van Tongeren.pdf - 3,472,000 bytesMD5:
ee9b21844d9c58b1a54075d3ecc8f082
oriTP 14-07-2020_spill_applied_events_Fig 3.zip - 2,443,354 bytesMD5:
bdecf6143d039a99428d1f55638f64bf
oriTP 29-07-2020_spill_applied_events_Fig 3.zip - 3,815,266 bytesMD5:
46e6f2771356d0553c6670a996c5b117
PSSA_Fig5 and Sup Figs on Fig 5.zip - 3,625,751 bytesMD5:
0eb0cdd980bb2b9b2657b6135be34fe5
PSSA_rep1_Fig5 and Sup Figs on Fig 5.zip - 149,806,560 bytesMD5:
3afaabb86d5b2cf4a7e14325ab55b449
PSSA_rep2_Fig5 and Sup Figs on Fig 5.zip - 6,421 bytesMD5:
a31ffa2dfbcecc6404bd286410bbaa80
Save_Recognized_liposomes_SMELDit MATLAB.m - 304,101 bytesMD5:
bec911bc26a2c2cf367de3d72d96bede
SnapGene_G338-pUC-OriL191-pT7-TP-T7t-OriR194.dna - 468,705 bytesMD5:
bb1712f6a6e72ebb716fb3de33380faa
SnapGene_G340-pUC57-OriL191pT7-pg2_DNAPphi29-vsv-altspacer2-TP-opt-T7t-OriR194.dna - 199,320 bytesMD5:
0fcd63055245c8e8e923babfc91b3b61
SnapGene_G365-OriL191pT7-YFPco-T7t-OriR194.dna - 170,585 bytesMD5:
990c5dbafb1c5a553038c911b948bf14
SnapGene_G368-OriL191pT7-PssA-T7t-OriR194.dna - 121,351 bytesMD5:
678b2fca23d6baa7274f4871e479cb3d
SnapGene_G85 - pUC57-T7p-DNAPphi29-GSopt-vsv.dna - 271,590 bytesMD5:
d05808ebf860e34d455c05cf07147c93
SnapGene_G88-pUC57-oriL191_gp6_DBP_phi29_ori194.dna - 79,406 bytesMD5:
5e6b18dd2b3ee845f9421097fd0ec454
SnapGene_G95-pUC57-OriL191pT7-pg2_DNAPphi29-vsv-TP-opt-T7t-OriR194.dna - 8,207,736 bytesMD5:
b1e5916e064ee1d0f853d12a80c69967
sorting oriMinD-oriYFP_spill_applied_events_Fig 4.zip - 1,191,065 bytesMD5:
023eab057f608924e7f5224feb779146
SupInfo_clonal-amplification-enhanced-gene-expression-in-synthetic-vesicles.pdf - 11,953 bytesMD5:
f9f0f348d70f852615c5c746869233e0
Suplementary Figures.prism -
download all files (zip)
3,133,929,018 bytes unzipped