Data underlying the publication: miRNA and mRNA integrated analysis reveal differential expression genes in the gonad of sea cucumber Holothuria leucospilota

DOI:10.4121/cd14b323-144d-4828-b2c3-1f465f74b710.v1
The DOI displayed above is for this specific version of this dataset, which is currently the latest. Newer versions may be published in the future. For a link that will always point to the latest version, please use
DOI: 10.4121/cd14b323-144d-4828-b2c3-1f465f74b710

Datacite citation style

Zhang, Jing; Fu, Xiaoqin; Lin, Runxin; Zhang, Yang; Zheng, Peiming et. al. (2025): Data underlying the publication: miRNA and mRNA integrated analysis reveal differential expression genes in the gonad of sea cucumber Holothuria leucospilota. Version 1. 4TU.ResearchData. dataset. https://doi.org/10.4121/cd14b323-144d-4828-b2c3-1f465f74b710.v1
Other citation styles (APA, Harvard, MLA, Vancouver, Chicago, IEEE) available at Datacite

Dataset

MicroRNAs are defined as a class of short noncoding RNAs, which play important function in cell differentiation, developmental process and gender determination. The sea cucumber Holothuria leucospilota is a member of the Echinodermata, known for its excellent nutritional and economic value. Breeding efficiency of sea cucumbers is low due to the difficulty in distinguishing between male and female individuals. Discovering molecular markers that distinguish between genders will provide a robust strategy for elucidating the genetic mechanisms governing gender determination and differentiation. In this investigation, high-throughput sequencing was utilized to compare miRNA expression patterns in the gonadal tissues of male and female specimens of Holothuria leucospilota. A total of 584 known miRNAs and 404 novel miRNAs were identified, of which 97 miRNAs were identified as significantly differentially expressed miRNAs (DEMs) between male and female sea cucumbers. These findings enabled the construction of a miRNA-mRNA regulatory network. Pathway analysis of target genes highlighted the potential roles of neuroactive ligand-receptor interactions, calcium signaling, Salmonella infection, endocytosis, and MAPK signaling in governing gener differentiation processes. The expression results of high-throughput sequencing were validated in sc-miR121, sc-miR41, sc-miR67, sc-miR90, sc-miR104 and sc-miR189 by quantitative real-time quantity polymerase chain reaction (RT-qPCR). In summary, our results will provide a foundation for future studies of miRNA regulation in gender differentiation of sea cucumber H. leucospilota.

History

  • 2025-09-25 first online, published, posted

Publisher

4TU.ResearchData

Format

.xlsx

Organizations

Guangdong Pharmaceutical University, School of Life Sciences and Biopharmaceutics

DATA

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