The doi above is for this specific version of this dataset, which is currently the latest. Newer versions may be published in the future. For a link that will always point to the latest version, please use
doi: 10.4121/c5f64852-b82d-41e5-9efc-1296e6698009

Humberto Sánchez; Liu, Zhaowei; Edo van Veen; Theo van Laar; John FX Diffley et. al. (2023): Data underlying the publication: “A chromatinized origin reduces the mobility of ORC and MCM through interactions and spatial constraint". Version 1. 4TU.ResearchData. dataset. https://doi.org/10.4121/c5f64852-b82d-41e5-9efc-1296e6698009.v1

Dataset

• Other Biological Sciences
• Biochemistry and Cell Biology
• Biological Sciences

chromatin, DNA replication, MCM, Mcm2-7/Cdt1, nucleosomes, ORC, protein diffusion, replication origin, target search, yeast replication

CC BY 4.0

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by Humberto Sánchez , Zhaowei Liu, Edo van Veen, Theo van Laar, John FX Diffley, Nynke H. Dekker

The aim of the research was to quantify the location and movement of the DNA replication factors ORC and MCM in the context of chromatin at the single-molecule level.

This was achieved using a combination of optical tweezers and confocal fluorescence microscopy. The position along DNA molecules held in an optical trap of fluorescently labelled H2A, ORC and MCM was tracked over time, and position and motion parameters (e.g. localization and diffusion constants) were studied under different biochemical conditions.

• 2023-10-16 first online, published, posted

4TU.ResearchData

Compressed zipped shape files

A chromatinized origin reduces the mobility of ORC and MCM through interactions and spatial constraint

• https://www.biorxiv.org/content/10.1101/2023.05.18.541303v3

• Netherlands Organization for Scientific Research (NWO) Top grant 714.017.002 (grant code 714.017.002) Netherlands Organization for Scientific Research
• BaSyC – Building a Synthetic Cell Gravitation grant (grant code 024.003.019) NWO
• ERC Advanced grant no. 789267, REPLICHROMA
• EMBO Postdoctoral Fellowship (ALTF 484-2022) (grant code ALTF 484-2022) EMBO

Department of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology.
Chromosome Replication Laboratory, Francis Crick Institute, London, UK.