Genome-Wide Identification of Conditionally Essential Genes Supporting Streptococcus suis Growth in Serum and Cerebrospinal Fluid – Supplementary Dataset
DOI: 10.4121/ace99cb5-0414-4a91-9aa8-72c0d8c47381
Datacite citation style
Dataset
This dataset contains supplementary data for the study “Genome-Wide Identification of Conditionally Essential Genes Supporting Streptococcus suis Growth in Serum and Cerebrospinal Fluid.” The research aimed to identify conditionally essential genes that facilitate S. suis survival in host environments. Using transposon sequencing (Tn-seq) combined with Oxford Nanopore sequencing, the study profiled mutant fitness in active porcine serum and cerebrospinal fluid derived from human choroid plexus organoids. The dataset includes Tn-seq output files, gene annotations, and raw and processed data supporting Figures 2 to 6 of the manuscript and Supplementary Tables S1 to S3. A detailed README file is included to describe the content and structure of each dataset component. The raw FAST5 and FASTQ sequencing files are available in the European Nucleotide Archive (ENA) under study accession number PRJEB89170. The Tn-Seq analysis pipeline developed for this study, Nanopore-TnSeq-Pipeline, is also available and can be accessed via a link provided in both the manuscript and this data repository.
History
- 2025-06-11 first online, published, posted
Publisher
4TU.ResearchDataFormat
.docx, .xlsx, .txtAssociated peer-reviewed publication
Genome-Wide Identification of Conditionally Essential Genes Supporting Streptococcus suis Growth in Serum and Cerebrospinal FluidFunding
- Horizon 2020 Marie Skłodowska-Curie grant agreement (grant code No. 956154)
- China Scholarship Council (grant code (No. 201906350084))
- Research Center for Genetic Engineering, BRIN, Indonesia
Organizations
Host-Microbe Interactomics, Animal Sciences, Wageningen University & ResearchDATA
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- 7,216 bytesMD5:
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README.txt - 681,552 bytesMD5:
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Supplementary Data S10 – P17_anotation file.txt - 9,059 bytesMD5:
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Supplementary Data S4 – Figure 2.xlsx - 23,262 bytesMD5:
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Supplementary Data S5 – Figure 3.xlsx - 9,111 bytesMD5:
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Supplementary Data S6 – Figure 4.xlsx - 47,666 bytesMD5:
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Supplementary Data S7 – Figure 6.xlsx - 193,348 bytesMD5:
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Supplementary Data S8 –TnseqAPS_transit output.xlsx - 187,775 bytesMD5:
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Supplementary Data S9 –TnseqCSF_transit output.xlsx - 26,479 bytesMD5:
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Table S1.docx - 31,941 bytesMD5:
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Table S2.docx - 29,692 bytesMD5:
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Table S3.docx -
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