Data underlying the publication: Preserving Full Spectrum Information in Imaging Mass Spectrometry Data Reduction

doi:10.4121/a6efd47a-b4ec-493e-a742-70e8a369f788.v1
The doi above is for this specific version of this dataset, which is currently the latest. Newer versions may be published in the future. For a link that will always point to the latest version, please use
doi: 10.4121/a6efd47a-b4ec-493e-a742-70e8a369f788
Datacite citation style:
Spraggins, Jeffrey M.; Van Ardenne, Jacqueline M. (2024): Data underlying the publication: Preserving Full Spectrum Information in Imaging Mass Spectrometry Data Reduction. Version 1. 4TU.ResearchData. dataset. https://doi.org/10.4121/a6efd47a-b4ec-493e-a742-70e8a369f788.v1
Other citation styles (APA, Harvard, MLA, Vancouver, Chicago, IEEE) available at Datacite
Dataset

FT-ICR

Human kidney tissue was surgically removed during a full

nephrectomy, and remnant tissue was processed for research

purposes by the Cooperative Human Tissue Network (CHTN)

at Vanderbilt University Medical Center. Human biospecimens

were collected in compliance with the CHTN protocols,

institutional IRB policies, and the National Cancer Institute’s

best practices for procurement of remnant surgical research

material. The tissue was flash-frozen over an isopentane-dry

ice slurry and embedded in carboxymethylcellulose. Tissue

sections were cryosectioned with a thickness of 10 μm and

thaw-mounted onto indium tin-oxide-coated glass slides (Delta

Technologies, Loveland, CO, USA). 1,5-Diaminonaphthalene

(DAN) was applied to the tissue surface using a TM Sprayer M3

(HTX Technologies, Chapel Hill, NC, USA). The sample was

imaged (50 μm pitch) directly after matrix application with

a 15T MALDI Fourier-transform ion cyclotron resonance (FT-

ICR) mass spectrometer (Solarix, Bruker Daltonics, Billerica,

MD, USA). Briefly, data were generated from m/z 300-2000

with a 4M file size in negative ion mode.


qTOF

C57BL6/J mice were retro-orbitally infected with S. aureus Newman and sacrificed humanely five days post-infection. Kidneys were flash frozen on an isopentane/dry ice slurry and embedded in 2.6\% carboxymethylcellulose. 5 μm thick sections were collected using a Leica Biosystems CM3050S cryostat and thaw-mounted onto indium tin oxide coated glass slides. Sections were washed with cold 150 mM ammonium formate for 45 seconds for three total washes. 5 mg of 4-(dimethylamino)cinnamic acid matrix was applied using an in-house sublimation device. MALDI IMS data were collected in negative ion mode from m/z 400-2000 using a Bruker MALDI timsTOF fleX platform with a 5 μm step size, 25% laser power, and 25 shots per pixel.

history
  • 2024-10-08 first online, published, posted
publisher
4TU.ResearchData
format
.d, .tsf, .tsf_bin, .mcf, .mcf_idx
organizations
Vanderbilt University; Mass Spectrometry Research Center;
TU Delft, Faculty of Mechanical Engineering, Delft Center for Systems and Control (DCSC)