Bloemendal, Victor; Moons, S.J. (Sam); Hemming, J.J.A. (Jurriaan); Chayoua, M. (Mohamed); Niesink, O. (Olaf) et. al. (2019): Data underlying the research of Chemoenzymatic Synthesis of Sialic Acid Derivatives Using Immobilized N-Acetylneuraminate Lyase in a Continuous Flow Reactor. Version 1. 4TU.ResearchData. dataset. https://doi.org/10.4121/uuid:74547c17-9760-4e07-b901-a6d9cd21d092

Dataset

• Medicinal and Biomolecular Chemistry
• Analytical Chemistry

Enzyme immobilization, Flow chemistry, N-acetylneuraminate lyase, N-acetylneuraminic acid, Sialic acids

CC BY-NC-SA 4.0

• Catalyst Cascade Reactions in ‘One-Flow’ within a Compartmentalized, Green-Solvent ‘Digital Synthesis Machinery’ – End-to-End Green Process Design for Pharmaceuticals.

RefWorks, BibTeX, Reference Manager, Endnote, DataCite, NLM, DC, CFF

by Victor Bloemendal , S.J. (Sam) Moons , J.J.A. (Jurriaan) Hemming , M. (Mohamed) Chayoua , O. (Olaf) Niesink , Jan van Hest , T.J. (Thomas) Boltje , F.P.J.T. (Floris) Rutjes

NMR research data related to a continuous flow process involving N‐acetylneuraminate lyase (NAL) immobilized on Immobead 150P to prepare Neu5Ac derivatives. Batch experiments with Immobead‐NAL showed equal activity as the native enzyme. By using a fivefold excess of either N‐acetyl‐D‐mannosamine (ManNAc) or pyruvate the conversion and isolated yield of Neu5Ac were significantly improved. To further increase the efficiency of the process, a flow setup was designed providing a chemoenzymatic entry into a series of N‐functionalized Neu5Ac derivatives in conversions of 48‐82%, and showing excellent stability over 1 week of continuous use.

• 2019-04-25 first online, published, posted

4TU.Centre for Research Data

media types: application/octet-stream, application/pdf, application/x-rar-compressed, text/plain, text/xml

• https://doi.org/10.1002/adsc.201900146
• https://mestrelab.com/software/mnova/

Eindhoven University of Technology;
Radboud University Nijmegen