cff-version: 1.2.0
abstract: "NMR research data related to a continuous flow process involving N‐acetylneuraminate lyase (NAL) immobilized on Immobead 150P to prepare Neu5Ac derivatives. Batch experiments with Immobead‐NAL showed equal activity as the native enzyme. By using a fivefold excess of either N‐acetyl‐D‐mannosamine (ManNAc) or pyruvate the conversion and isolated yield of Neu5Ac were significantly improved. To further increase the efficiency of the process, a flow setup was designed providing a chemoenzymatic entry into a series of N‐functionalized Neu5Ac derivatives in conversions of 48‐82%, and showing excellent stability over 1 week of continuous use."
authors:
  - family-names: Bloemendal
    given-names: Victor
    orcid: "https://orcid.org/0000-0003-0013-6015"
  - family-names: Moons
    given-names: S.J. (Sam)
  - family-names: Hemming
    given-names: J.J.A. (Jurriaan)
    orcid: "https://orcid.org/0000-0002-9217-3616"
  - family-names: Chayoua
    given-names: M. (Mohamed)
  - family-names: Niesink
    given-names: O. (Olaf)
  - family-names: van Hest
    given-names: Jan
    orcid: "https://orcid.org/0000-0001-7973-2404"
  - family-names: Boltje
    given-names: T.J. (Thomas)
    orcid: "https://orcid.org/0000-0001-9141-8784"
  - family-names: Rutjes
    given-names: F.P.J.T. (Floris)
    orcid: "https://orcid.org/0000-0003-1538-3852"
title: "Data underlying the research of Chemoenzymatic Synthesis of Sialic Acid Derivatives Using Immobilized N-Acetylneuraminate Lyase in a Continuous Flow Reactor"
keywords:
version: 1
identifiers:
  - type: doi
    value: 10.4121/uuid:74547c17-9760-4e07-b901-a6d9cd21d092
license: CC BY-NC-SA 4.0
date-released: 2019-04-25