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DOI: 10.4121/730912b4-106d-473d-95e5-33125ec81e26

van Noord, Aster (2025): Data supplementary to the PhD Dissertation: Driving asymmetric biocatalysis through cofactor regeneration. Version 1. 4TU.ResearchData. dataset. https://doi.org/10.4121/730912b4-106d-473d-95e5-33125ec81e26.v1

Categories

• Other Chemical Sciences
• Biochemistry and Cell Biology
• Biological Sciences
• Chemical Sciences

Keywords

asymmetric synthesis biocatalysis cofactor regeneration epoxidation flavin adenine dinucleotide halide methyltransferases hydrogenases S-adenosyl-L-methionine analogues styrene monooxygenases

Licence

CC BY-NC-ND 4.0

Interoperability

• RO-Crate Metadata

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by Aster van Noord

The enclosed data is complementary to the dissertation “Driving asymmetric biocatalysis through cofactor regeneration”. The aim of the dissertation is to promote biocatalytic asymmetric alkylation and epoxidation by exploring the regeneration of their associated cofactors. To drive asymmetric alkylation, we employed protein engineering to expand the substrate scope of the halide methyltransferase from Arabidopsis thaliana to regenerate the cofactor S-Adenosyl-L-homocysteine with alkyl groups lager than methyl. Asymmetric epoxidation was explored by characterisation of the substrate scope of styrene monooxygenases, and by recycling their cofactor flavin adenine dinucleotide (FAD) in a clean fashion by hydrogenases. The raw data for the figures and tables are sorted per chapter in the accompanying .zip file. For more details on the research objective, type of research and method of data collection, please go to README file provided per chapter.

History

• 2025-09-16 first online, published, posted

Publisher

4TU.ResearchData

Format

Excel (.xlsx), Powerpoint (.pptx)

Organizations

TU Delft, Faculty of Applied Sciences, Department of Biotechnology, Biocatalysis section