Supplementary materials for PhD thesis: Comparative Genomics and Trait Evolution in Lettuce, its Wild Relatives and the Asteraceae
DOI: 10.4121/21803436
Dataset
Usage statistics
Licence CC BY 4.0
This dataset includes the supplementary materials of Wei Xiong's Ph.D. thesis book for different chapters. It contains the evidence that supports the main findings or the important intermediate results. The work was conducted in the Biosystematics group, at Wageningen University
Improved crops are needed to provide healthy and nutritious food for the growing global population. Genome assembly helps understand genetic diversity and chromosome organization, which is crucial for breeding improved crops.
This study focuses on lettuce (Lactuca sativa) and its wild relatives, which serve as important genetic resources for breeding. Chapter 1 discusses sequencing technologies for genome assembly and introduces three key species for lettuce breeding: L. saligna, L. virosa, and dandelion (Taraxacum officinale).
L. saligna belongs to lettuce’s secondary gene pool and provides resistance to downy mildew. Chapter 2 presents a high-quality genome assembly of L. saligna, describing genetic variants, structural differences compared to lettuce, and potential genetic barriers in hybridization.
L. virosa is part of the tertiary gene pool and is naturally difficult to cross with lettuce. Chapter 3 details the genome assembly of L. virosa and compares its genetic structure with L. saligna and L. sativa, identifying major structural differences and repetitive elements.
Chapter 4 introduces a genome assembly of diploid dandelion as a reference for lettuce breeding and a phylogenomic study within the Asteraceae family. The evolution of resistance and floral traits is explored, with a focus on MADS-box genes.
Chapter 5 provides a synthesizing discussion and perspectives for future research, including improved genome assembly strategies, the use of model plants, and the role of specific genes in disease resistance and flower development. This study contributes to genetic knowledge and breeding possibilities for lettuce species and related crops. (Summary by ChatGPT)
History
- 2025-02-28 first online, published, posted
Publisher
4TU.ResearchDataFormat
xlsx and docOrganizations
Biosystematics group, Wageningen University & ResearchDATA
Files (23)
- 6,659 bytesMD5:
000c5c20e400eb47b2bf9ae6e7394180
README.txt - 14,299 bytesMD5:
be772e5dc3f89e87c5c69a62d833f978
2_PhD_thesis_Chapter2_SupplementalDS1.xlsx - 1,998,510 bytesMD5:
5f52f37ee16d813ae5da28ed06c0fdfc
2_PhD_thesis_Chapter2_SupplementalDS2A-F.xlsx - 1,073,945 bytesMD5:
ccc93755f995ac7560ce0687a3b34bf0
2_PhD_thesis_Chapter2_SupplementalDS3A-B.xlsx - 5,559,224 bytesMD5:
119919ba3f40002393a0f950f17d6d38
2_PhD_thesis_Chapter2_SupplementalDS4A-B.xlsx - 143,171 bytesMD5:
3daf0ad25f2c0fce26aba22afecf0df4
2_PhD_thesis_Chapter2_SupplementalDS5A-D.xlsx - 358,285 bytesMD5:
65735cb41d6bf760a2fc18cf11b61eef
2_PhD_thesis_Chapter2_SupplementalDS6A-B.xlsx - 3,686,939 bytesMD5:
e5f065b3cc2727de0a1b42e72edb2d04
2_PhD_thesis_Chapter2_SupplementalDS7.xlsx - 3,606,657 bytesMD5:
bd370bf75fae26b6fdfb6495f0b18355
2_PhD_thesis_Chapter2_Supplmentary-Figures-Tables.docx - 2,684,898 bytesMD5:
ae9fc85d46d224ad2d6586bfe401f695
2_PhD_thesis_Chapter2_Supplmentary-Note.docx - 3,981,618 bytesMD5:
abeb8a95187b95c885c630f114516ce1
3_PhD_thesis_Chapter3_Supplementary-Figures-Tables.docx - 292,584 bytesMD5:
faa93e4926e6f00898fc2f101201d94c
3_PhD_thesis_Chapter3_Supplementary_Data_1 - Genome assembly and scaffolding.xlsx - 4,598,102 bytesMD5:
e023e085dad91e26ea490ff7024490b2
3_PhD_thesis_Chapter3_Supplementary_Data_2 - Functional annotation and homology grouping of L. virosa transcripts.xlsx - 87,971 bytesMD5:
5c4d0fa2d81395eb818e640ca4e41983
3_PhD_thesis_Chapter3_Supplementary_Data_3 - Repeatome analysis of L. virosa, L. sativa and L. saligna.xlsx - 433,229 bytesMD5:
f0988851a70cccbf14477f9dccb6e9a7
3_PhD_thesis_Chapter3_Supplementary_Data_4 - Identified NLR and RLK proteins in L. virosa and L. saligna.xlsx - 156,392 bytesMD5:
e7a8ba3be5c95656a515d5acaaec5c50
3_PhD_thesis_Chapter3_Supplementary_Data_5 - Overview of homology within NLRs and RLKs in Lactuca.xlsx - 6,194,806 bytesMD5:
3287c45952c4d78b181858d43b1c7bec
4_PhD_thesis_Chapter4_Supplementary-Figures.docx - 714,755 bytesMD5:
6bbfa34f2576d4b1e2982f14a4c60bf7
4_PhD_thesis_Chapter4_Supplementary-Tables.xlsx - 11,353,269 bytesMD5:
e253db0bc81655264b8efc676f66dd13
4_PhD_thesis_Chapter4_Supplementary_Data_1 - Gene description.xlsx - 151,138,918 bytesMD5:
3c83561275bb001f326fbde0c41b6434
4_PhD_thesis_Chapter4_Supplementary_Data_2 - Gene expression.xlsx - 223,925 bytesMD5:
3e2a915f72404f6d6e0bebfd6881c9bb
4_PhD_thesis_Chapter4_Supplementary_Data_3 - Gene_identify.xlsx - 548,619 bytesMD5:
5e2bb6474d077e875e66fd01f8a80605
4_PhD_thesis_Chapter4_Supplementary_Data_4 - SynNet-of-target-genes.xlsx - 32,951 bytesMD5:
5c9015a24f0e1c16c1fc4ae0b9fc0d81
4_PhD_thesis_Chapter4_Supplementary_Data_5 - Tandem-Duplicates-of-target-genes.xlsx -
download all files (zip)
198,889,726 bytes unzipped