Data underlying the publication: “Nucleotide binding halts diffusion of the eukaryotic replicative helicase during activation”

doi: 10.4121/19948253.v1
The doi above is for this specific version of this dataset, which is currently the latest. Newer versions may be published in the future. For a link that will always point to the latest version, please use
doi: 10.4121/19948253
Datacite citation style:
Nynke Dekker; Daniel Ramirez Montero; Edo van Veen; Humberto Sánchez; Theo van Laar et. al. (2023): Data underlying the publication: “Nucleotide binding halts diffusion of the eukaryotic replicative helicase during activation”. Version 1. 4TU.ResearchData. dataset. https://doi.org/10.4121/19948253.v1
Other citation styles (APA, Harvard, MLA, Vancouver, Chicago, IEEE) available at Datacite
Dataset

The research objective was to quantify at the single-molecule level the motion of fully in vitro reconstituted CMG, the eukaryotic replicative helicase. 


This was achieved by using a combination of optical tweezers and confocal fluorescence microscopy. The position along DNA molecules held in an optical trap of fluorescently labeled CMG was tracked over time, and different motion parameters were studied (e.g. diffusion constants and velocities) under different biochemical conditions.

history
  • 2023-02-03 first online, published, posted
publisher
4TU.ResearchData
format
csv, yaml, TIFF
funding
  • Boehringer Ingelheim Fonds PhD Fellowship
  • Netherlands Organisation for Scientific Research (NWO) Top grant 714.017.002
  • European Research Council through an Advanced Grant (REPLICHROMA; grant number 789267)
organizations
TU Delft, Faculty of Applied Sciences, Kavli Institute of Nanoscience, Department of Bionanoscience

DATA

files (1)