TY - DATA
T1 - Data underlying the publication: “Nucleotide binding halts diffusion of the eukaryotic replicative helicase during activation”
PY - 2023/02/03
AU - Nynke Dekker
AU - Daniel Ramirez Montero
AU - Edo van Veen
AU - Humberto Sánchez
AU - Theo van Laar
AU - Belen Solano Hermosilla
AU - John FX Diffley
UR - https://data.4tu.nl/articles/dataset/Data_underlying_the_publication_Nucleotide_binding_halts_diffusion_of_the_eukaryotic_replicative_helicase_during_activation_/19948253/1
DO - 10.4121/19948253.v1
KW - DNA replication initiation
KW - Single-molecule biophysics
KW - Enzyme mechanisms
KW - Eukaryotic DNA replication
N2 - <p>The research objective was to quantify at the single-molecule level the motion of fully in vitro reconstituted CMG, the eukaryotic replicative helicase. </p>
<p><br></p>
<p>This was achieved by using a combination of optical tweezers and confocal fluorescence microscopy. The position along DNA molecules held in an optical trap of fluorescently labeled CMG was tracked over time, and different motion parameters were studied (e.g. diffusion constants and velocities) under different biochemical conditions.</p>
ER -