The doi above is for this specific version of this dataset, which is currently the latest. Newer versions may be published in the future. For a link that will always point to the latest version, please use
doi: 10.4121/5e6b4b53-6188-4254-a729-47991bbb8832

Buellesbach, Jan; Lammers, Mark; van de Belt, Jose; Pannebakker, Bart (2023): Chemical and population data for Nasonia vitripennnis females collected from the Hoge Veluwe. Version 1. 4TU.ResearchData. dataset. https://doi.org/10.4121/5e6b4b53-6188-4254-a729-47991bbb8832.v1

Dataset

• Ecology
• Genetics
• Evolutionary Biology
• Zoology
• Biological Sciences

chemical ecology, Cuticular hydrocarbons, microsatellites, niche differentiation, parasitoid wasps, speciation

2018

CC BY 4.0

RefWorks, BibTeX, Reference Manager, Endnote, DataCite, NLM, DC, CFF

by Jan Buellesbach , Mark Lammers , Jose van de Belt , Bart Pannebakker

Abstract

Ecotypes, strains locally adapted to divergent ecological conditions, are often considered to be the first steps in sympatric speciation. It has been suggested that two distinguishable ecotypes hint at incipient sympatric speciation in Nasonia vitripennis, the prominent model organism for parasitoid wasps,  with one ecotype parasitizing fly pupae in bird nests, and the other parasitizing fly pupae on carrion. In the present study, we investigated the differentiation into these two distinct ecotypes on a population genetic and on a phenotypic level in a wild N. vitripennis population in the Netherlands. Isofemale lines were obtained from bird nest boxes and from deer carrion, respectively, representing both microhabitats. Using a panel of 14 microsatellites, we determined the population genetic structure and tested for genetic differentiation between the foundresses obtained from both microhabitats. To test for phenotypic differentiation, we determined the cuticular hydrocarbon (CHC) profiles from wasps of both microhabitats. Both the genetic and the phenotypic datasets show no evidence for any kind of separation based on the postulated two ecotypes, but rather suggest free interbreeding with no gene flow interruption between the two distinct host patches. This challenges previous assumptions on distinguishable ecotypes in N. vitripennis, and invites a re-evaluation of potential ecological speciation mechanisms in parasitoid wasps.

This dataset contains:

1) microsatellite data for 14 loci 25 N. vitripennis females collected in 2018 in the Hoge Veluwe in The Netherlands (HV2018_MicrosatelliteDataset.csv)

2) Raw data of GC-MS profiles of cuticular hydrocarbon (CHC) of N.vitripennis males and females from iso-female lines collected in 2018 in the Hoge Veluwe in The Netherlands (HV2018_CHC_RawData.tar) and an overview (HV2018_GCMS_Data.csv).

• 2023-11-10 first online, published, posted

4TU.ResearchData

csv

• German Research Foundation to Jan Buellesbach (DFG, BU3439/1-1) (grant code DFG, BU3439/1-1) German Research Foundation

Institute for Evolution & Biodiversity, University of Münster, Germany;
Laboratory of Genetics, Wageningen University & Research.