The doi above is for this specific version of this dataset, which is currently the latest. Newer versions may be published in the future. For a link that will always point to the latest version, please use
doi: 10.4121/5bb846d8-ed30-4e2e-bfe3-4fb607cbe8ed

Weener, Huub; van Haaps, Thijs; van Helden, Ruben; Albers, Hugo; Haverkate, Rozemarijn et. al. (2024): Data of manuscript Blood-perfused Vessels-on-Chips stimulated with patient plasma recapitulate endothelial activation and microthrombosis in COVID-19. Version 1. 4TU.ResearchData. dataset. https://doi.org/10.4121/5bb846d8-ed30-4e2e-bfe3-4fb607cbe8ed.v1

Dataset

• Clinical Sciences
• Biomedical Engineering
• Biochemistry and Cell Biology
• Engineering
• Biological Sciences
• Medical and Health Sciences

Clinical data, Corona, COVID-19, Cytokine, ELISA, Organ-on-Chip, RNA sequencing, Thrombosis

01-01-2021 to 20-05-2021

CC BY-NC 4.0

RefWorks, BibTeX, Reference Manager, Endnote, DataCite, NLM, DC, CFF

by Huub Weener , Thijs van Haaps , Ruben van Helden , Hugo Albers , Rozemarijn Haverkate , Heleen Middelkamp , Milan Ridderikhof , Thijs van Mens , Albert van den Berg , Christine Mummery , Valeria Orlova , Saskia Middeldorp , Nick van Es , Andries van der Meer

This dataset contains the data for the manuscript "Blood-perfused Vessels-on-Chips stimulated with patient plasma recapitulate endothelial activation and microthrombosis in COVID-19", to be published. Within the data are multiple sets:

• clinical data from included patients and controls; including admission time in hospital, diagnostic measurements when first included, outcome of admission, and VTE status during admission
• Cytokine ELISAs of IL-6, IL-10, TNF-a, IFN-y, CXCL-10, measured in the derived plasma of hospitalized patients
• Raw reads of RNA sequencing of hiPSC derived endothelial cells, after cells were stimulated with diluted patient plasma overnight to induce changes in their phenotype
• Raw pathogen counts based on said RNA sequencing data, where non-human RNA reads are quantified per sample
• Platelet coverage data after hiPSC-ECs were stimulated with derived plasma on-chip, where the stimulated chips were perfused with human whole blood to recapitulate the thrombotic adverse events that occur in some COVID patients.

• 2024-09-17 first online, published, posted

4TU.ResearchData

.csv

• Netherlands Organ-on-Chip Initiative (grant code 024.003.001) [more info...] NWO Graviation
• imSAVAR (grant code 853988) [more info...] EU/EFPIA/JDRF INTERNATIONAL Innovative Medicines Initiative 2
• ERC advanced grant VESCEL (grant code 669768) [more info...] European Research Council
• ERC advanced STEMCARDIOVASC (grant code 323182) [more info...] European Research Council
• ZonMw COVID-19 MKMD program (grant code 114025010) [more info...] ZonMw

University of Twente, Faculty of Science and Technology (TNW), Bioengineering Technologies (TNW-BET);
Amsterdam University Medical Center, Department of Vascular Medicine;
Leiden University Medical Center, Department of Anatomy and Embryology;
Radboud University Medical Center, Department of Internal Medicine