Data underlying the publication: The effects of thioredoxin peroxidase from Cysticercus cellulosae excretory-secretory antigens on TGF-β signaling pathway and Th17 cells differentiation in Jurkat cells by transcriptomics
Translated Chinese text by ChatGPT:
Background: The sulfur oxygen reductase peroxidase (TPx) protein from Cellulosimicrobium cellulans excretory-secretory antigen (ESA) has been shown to regulate the differentiation of host Treg and Th17 cells, resulting in an immune suppression response dominated by Treg cells. However, the molecular mechanisms by which the TPx protein from Cellulosimicrobium cellulans ESA regulates the differentiation of host Treg/Th17 cells have not been reported.
Methods: Jurkat cells stimulated with TPx protein from porcine Cellulosimicrobium cellulans ESAs at 0, 24, 48, and 72 hours with PMA and ionomycin activation. Transcriptomic analysis was performed to study the signaling pathways related to Jurkat cell differentiation regulated by TPx protein from porcine Cellulosimicrobium cellulans ESA.
Results: Gene Set Enrichment Analysis (GSEA) showed that TPx protein from porcine Cellulosimicrobium cellulans ESAs can induce upregulation of the TGF-β signaling pathway in Jurkat cells and downregulation of Th17 cell differentiation.
Conclusion: The TPx protein from porcine Cellulosimicrobium cellulans ESA can activate the TGF-β signaling pathway in Jurkat cells, thereby regulating the differentiation of Treg/Th17 cells, resulting in an immune suppression response dominated by Treg cells and evading host immune attacks. This study provides a foundation for further validating these pathways and elucidating the molecular mechanisms of immune escape caused by porcine Cellulosimicrobium cellulans.
- 2023-10-20 first online, published, posted
- the National Natural Science Foundation of China (grant code No. 81960378) Zhou Biying
- Basic Research Plan of Department of Science and Technology of Guizhou Province (grant code ZK general 516)