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DOI: 10.4121/19008833

Koen Verhagen; Eerden, Siem; S.A. (Aljoscha) Wahl (2022): Dataset proteomics: Analysis of change in protein expression in Saccharomyces cerevisiae upon shift from glucose chemostat to feast/famine regime. Version 1. 4TU.ResearchData. dataset. https://doi.org/10.4121/19008833.v1

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Categories

• Biochemistry and Cell Biology

Keywords

CEN.PK113-7D Dynamic conditions feast/famine Proteomics Saccharomyces cerevisiae

Licence

CC0

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by Koen Verhagen , Siem Eerden, S.A. (Aljoscha) Wahl

This proteomics dataset was generated to analyse the changes in protein expression between a chemostat and feast/famine regime culture of Saccharomyces cerevisiae grown on glucose at D = 0.1 h^-1. Proteome composition of the taken samples was subsequently measured by DSM, and the obtained dataset was analysed for significant changes in protein expression between chemostat and feast/famine conditions.
Files in this item:1) Materials and Methods:Materials_methods_proteomics_glucose_chemostat_FF_shift_Verhagen.docx
Describes the methodology of the reactor experiment, sampling and proteomics data analysis.
2) Proteomics data:Data_proteomics_glucose_shift_chemostat_FF_Verhagen.xlsx
Contains raw data received from DSM's proteomics measurements facility and subsequent statistical analysis.Read sheet 'Description' for explanation of other available sheets in the file.

History

• 2022-01-28 first online, published, posted

Publisher

4TU.ResearchData

Format

Excel (.xlsx) and Word (.docx) files

Funding

• NWO Building Blocks of Life 737.016.001

Organizations

Delft University of Technology, Faculty of Applied Sciences, Department of Biotechnology