Dataset proteomics: Analysis of change in protein expression in Saccharomyces cerevisiae upon shift from glucose chemostat to feast/famine regime

dataset

posted on 28.01.2022, 12:07 authored by Koen VerhagenKoen Verhagen, Siem Eerden, S.A. (Aljoscha) Wahl

This proteomics dataset was generated to analyse the changes in protein expression between a chemostat and feast/famine regime culture of Saccharomyces cerevisiae grown on glucose at D = 0.1 h^-1. Proteome composition of the taken samples was subsequently measured by DSM, and the obtained dataset was analysed for significant changes in protein expression between chemostat and feast/famine conditions.

Files in this item:

1) Materials and Methods:

Materials_methods_proteomics_glucose_chemostat_FF_shift_Verhagen.docx

Describes the methodology of the reactor experiment, sampling and proteomics data analysis.

2) Proteomics data:

Data_proteomics_glucose_shift_chemostat_FF_Verhagen.xlsx

Contains raw data received from DSM's proteomics measurements facility and subsequent statistical analysis.

Read sheet 'Description' for explanation of other available sheets in the file.

Funding

NWO Building Blocks of Life 737.016.001

History

Publisher

4TU.ResearchData

Format

Excel (.xlsx) and Word (.docx) files

Organizations

Delft University of Technology, Faculty of Applied Sciences, Department of Biotechnology

Usage metrics

Delft University of Technology

Keywords

Proteomics Saccharomyces cerevisiae feast/famine CEN.PK113-7D Dynamic conditions

Licence

CC0