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Data underlying the research on binding of archaeal histones HMfA and HMfB to DNA.

dataset
posted on 27.11.2020, 10:11 by Bram Henneman, Thomas Brouwer, Amanda M. Erkelens, Gert-Jan Kuijntjes, Clara van Emmerik, Ramon A. van der Valk, Monika Timmer, Nancy C.S. Kirolos, Hugo van Ingen, John van Noort, Remus T. Dame
These data were obtained in studies on hypernucleosome formation, an apparently endless nucleosome, by archaeal histones HMfA and HMfB from M. fervidus. Using tethered particle motion and magnetic tweezers the structural, mechanical as well as topological properties were investigated. In addition to the wild type proteins, derivatives mutated at residues proposed to be involved in 'stacking' interactions between layers of the hypernucleosome, were investigated; the data confirmed the importance of these residues.

Funding

NWO VICI 016.160.613/533

Human Frontier Science Program RGP0014/2014

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Publisher

4TU.ResearchData

Organizations

Leiden University Utrecht University

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