The DOI displayed above is for this specific version of this dataset, which is currently the latest. Newer versions may be published in the future. For a link that will always point to the latest version, please use
DOI: 10.4121/22004108

Chen, Xinlei (2023): Data underlying the research of: The mRNA expression of CACYBP in bladder and kidney cancer. Version 1. 4TU.ResearchData. dataset. https://doi.org/10.4121/22004108.v1

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Categories

• Medical Physiology
• Other Medical and Health Sciences
• Biomedical Engineering
• Medical Microbiology

Keywords

Calcyclin Binding Protein nephrology polymerase chain reaction (PCR) Siah-1 interacting protein (SIP） urology

Licence

CC0

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by Xinlei Chen

qRT-PCR 

Cellular RNA was extracted using Trizol, and total RNA (500 ng) was transcribed into cDNA using the PrimeScript kit. Actin primers were used as an internal control. Real-time fluorescence quantitative RT-PCR assays were performed using Lightcycler 480ii. The qRT-PCR primer sequences used in this study are shown below: Cacybp: forward primer: 5-CTCCCATTACAACGGGCTATAC-3, reverse primer: 5-GAACTGCCTTCCACAGAGATG-3; hactin: forward primer: 5-GGCATCGTCACCAACTGGGAC-3; reverse primer: 5-CGATTTCCCGCTCGGCCGTGG-3. Obtain the Cq datas of the actin and CACYBP gene of HK-2, O-786, SV-HUC-1, T24 cells  for analysis.

History

• 2023-02-08 first online, published, posted

Publisher

4TU.ResearchData

Format

*.xls

Organizations

The Affiliated Hospital of Qingdao University, Department of Urology, China