cff-version: 1.2.0
abstract: "<p><strong>qRT-PCR </strong></p>
<p>Cellular RNA was extracted using Trizol, and total RNA (500 ng) was transcribed into cDNA using the PrimeScript kit. Actin primers were used as an internal control. Real-time fluorescence quantitative RT-PCR assays were performed using Lightcycler 480ii. The qRT-PCR primer sequences used in this study are shown below: Cacybp: forward primer: 5-CTCCCATTACAACGGGCTATAC-3, reverse primer: 5-GAACTGCCTTCCACAGAGATG-3; hactin: forward primer: 5-GGCATCGTCACCAACTGGGAC-3; reverse primer: 5-CGATTTCCCGCTCGGCCGTGG-3. Obtain the Cq datas of the actin and CACYBP gene of HK-2, O-786, SV-HUC-1, T24 cells  for analysis.</p>"
authors:
  - family-names: Chen
    given-names: Xinlei
title: "Data underlying the research of: The mRNA expression of CACYBP in bladder and kidney cancer."
keywords:
version: 1
identifiers:
  - type: doi
    value: 10.4121/22004108.v1
license: CC0
date-released: 2023-02-08