The doi above is for this specific version of this dataset, which is currently the latest. Newer versions may be published in the future. For a link that will always point to the latest version, please use
doi: 10.4121/dbac218d-fca8-4c53-b39a-b7ebed3344ea

Oh, Jeong-Joo; Ammu, Satya; Vriend, Vivian; Kieffer, Roland; Kleiner, Friedrich et. al. (2023): Data underlying the publication: Growth, Distribution, and Photosynthesis of Chlamydomonas reinhardtii in 3D Hydrogels. Version 1. 4TU.ResearchData. dataset. https://doi.org/10.4121/dbac218d-fca8-4c53-b39a-b7ebed3344ea.v1

Dataset

• Other Biological Sciences
• Biological Sciences

3D printing, CO2 consumption, engineered living material, functional living surface, hydrogel, Microalgae, spatial organization

CC0

RefWorks, BibTeX, Reference Manager, Endnote, DataCite, NLM, DC, CFF

by Jeong-Joo Oh, Satya Ammu, Vivian Vriend, Roland Kieffer, Friedrich Kleiner, Srikkanth Balasubramanian, Elvin Karana, Kunal Masania, Marie-Eve Aubin-Tam

Engineered living materials (ELMs) are a novel class of functional materials that typically feature spatial confinement of living components within an inert polymer matrix to recreate biological functions. Understanding the growth and spatial configuration of cellular populations within a matrix is crucial to predicting and improving their responsive potential and functionality. Here, we investigate the growth, spatial distribution, and photosynthetic productivity of eukaryotic microalga Chlamydomonas reinhardtii in three-dimensionally shaped hydrogels in dependence of geometry and size. The embedded C. reinhardtii cells photosynthesize and form confined cell clusters, which grow faster when located close to the ELM periphery due to favorable gas exchange and light conditions. Taking advantage of location-specific growth patterns, we successfully design and print photosynthetic ELMs with increased CO₂ capturing rate, featuring high surface to volume ratio. This strategy to control cell growth for higher productivity of ELMs resembles the already established adaptations found in multicellular plant leaves.

• 2023-09-20 first online

• 2023-10-27 published, posted

4TU.ResearchData

zipped folder containing raw image files (.nd2, .bmp, .png, .jpg), numerical data (.xlsx), and design file (.stl)

Data underlying the publication: Growth, Distribution, and Photosynthesis of Chlamydomonas reinhardtii in 3D Hydrogels

• AlgaeLeaf (grant code NO.101042612) European Research Council
• Basic Science Research Program (grant code NRF-2022R1A6A3A03072013) National Research Foundation of Korea (NRF)

Kavli Institute of Nanoscience, Department of Bionanoscience, Delft University of Technology
TU Delft, Faculty of Industrial Design Engineering, Department of Sustainable Design Engineering
TU Delft, Faculty of Aerospace Engineering, Shaping Matter Lab, Faculty of Aerospace Engineering