Data underlying the research of the Role of bacterial proteins in the onset of ANCA vasculitis

doi: 10.4121/17171069.v1
The doi above is for this specific version of this dataset, which is currently the latest. Newer versions may be published in the future. For a link that will always point to the latest version, please use
doi: 10.4121/17171069
Datacite citation style:
Frans Bianchi; Peter Heeringa; Myrthe Frans; Anna van der Veen; Jildau van der Ven (2022): Data underlying the research of the Role of bacterial proteins in the onset of ANCA vasculitis. Version 1. 4TU.ResearchData. dataset.
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Goal: The aim of the project was to investigate whether S.aureus proteins SPIN and 6PGD via the formation of the hybrid auto-antigen SPIN:MPO or MPO mimitope 6PGD could explain the differential aetiologies of the disease GPA (Granulomatosis with polyangitis) caused by MPO-ANCA (Anti Neutrophil Cytoplasmic Autoantibodies).
Methods: Isolation kinetic verification of S.Aureus 6PGD and SPIN by means of Activity measurements, see Readme for further specifications.
Determination of antibody reactivity/titers using ELISA in sera from patients with MPO ANCA and healthy individuals against the hybrid auto-antigen MPO:ANCA or 6PGD, see Readme for further specifications.
Data: Antibody titers ELISA and (Kinetics) colorigenic activity assays.

  • 2022-01-18 first online, published, posted
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  • ZONMW OffRoad (04510011910005)
University of Groningen, Faculty of Science and Engineering

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