Data underlying the publication: Beetroot and Spinach Seed Microbiomes Can Suppress Pythium ultimum Infection; Results from a Large-Scale Screening
doi: 10.4121/20004842
CC BY-NC-ND 4.0
,
Liesbeth van der Heijden
,
Jorge Giovanny Lopez-Reyes
,
Anita van Nieuwenhoven
,
Martje Notten
,
Mirjam Storcken
,
Patrick Butterbach
,
Jürgen Köhl
,
Wietse de Boer
,
Joeke Postma
Seed health is an indispensable prerequisite of food security. While the toolkit of plant protection products is currently limited, evidence suggests that the seed microbiome could protect seeds from pathogens. Thus, given their possible disease suppressive potential, we tested 11 different pathosystems to achieve the following proof-of-concept: seed microbiomes can be beneficial for seed health through conferring disease suppression. This study focused on beetroot, onion, spinach, pepper, coriander, red fescue and perennial ryegrass seeds, with each crop being challenged with one or two from a total of six pathogens, namely Pythium ultimum (or a Pythium sp.), Setophoma terrestris, Fusarium oxysporum, Phytophthora capsici, Laetisaria fuciformis and a mix of Puccinia sp. isolates. Each seed lot of each crop was tested with and without treatment with a disinfectant as a proxy for comparing intact seed microbiomes with seed microbiomes after partial elimination by disinfection. We found disease suppression in two pathosystems. Beetroot and spinach seed lots were able to suppress disease caused by P. ultimum when their microbiomes were intact but not after seed disinfection. We speculate that this relates to the microorganisms residing on and in the seed. Yet, seed microbiome disease suppression was not found in all pathosystems, highlighting the variation in seed morphology, plant cultivars, pathogens and seed disinfection treatments. A holistic understanding of the characteristics of seeds that harbour suppressive microbiomes as well as the pathogens that are sensitive to suppression, could lead to more targeted and informed seed processing and treatment and, consequently, to the sustainable management of seedling diseases.
- 2022-07-10 first online
- 2022-08-25 published, posted
- LWV19097; Top Sector Horticulture and Propagation Materials
Soil Biology Group, Wageningen University and Research, The Netherlands
Bejo Zaden B.V., Warmenhuizen, The Netherlands
DLF B.V., Kapelle, The Netherlands
Pop Vriend Seeds B.V., Andijk, The Netherlands
Nunhems Netherlands B.V. (BASF), The Netherlands
CN seeds Ltd., United Kingdom
Germains Seed Technology, The Netherlands
Department of Microbial Ecology, Netherlands Institute of Ecology, Wageningen, the Netherlands
DATA
- 3,243 bytesMD5:
1fb874d63c35605125a4c89bbe737361README_Diakaki_et_al.txt - 21,963 bytesMD5:
173a957d3e81d2ea477af02b594a6745.Rhistory - 35,591 bytesMD5:
7aa42e93394898a6b6d792f8d1a792e201 data analysis.R - 7,000 bytesMD5:
3032f356bc0e28354935b209ed441e1201b data dispersion.R - 16,065 bytesMD5:
66f19e5059fe1a1bb40b0d555ae7a06602 data analysis.R - 6,450 bytesMD5:
3033a061e82b4ec3277ebf2e76a57fc103 data analysis and Fig.2.R - 2,667 bytesMD5:
61e2490ee817c19cfeda87d053f3f69704 make Fig.1.R - 26,487 bytesMD5:
98b46570708c0e4466798c03d590f22d05a make Fig.3.R - 23,919 bytesMD5:
e96ff31bbf517514feb78bfb7bf596cc05b make Fig.3.R - 19,135 bytesMD5:
16ca6650a2f41f22c4a65a728ac6158e06a make Suppl.Fig.1 continuous.R - 25,667 bytesMD5:
633e1062a23847b1215a71bc0ceb974a06b make Suppl.Fig.1 ordinal.R - 2,797 bytesMD5:
5e7bd9aeac9d0e98649d9ce1f4d6061506c combine Suppl.Fig.1.R - 7,363 bytesMD5:
1f7c00308f9b215b8a26c08c950c4477Beetroot4R_data.csv - 7,081 bytesMD5:
327b0c13cb342ce82b4925baf14a0ea2Beetroot_4Rgermination.csv - 7,444 bytesMD5:
70241e0fed2fb3cfb9ce1f3e4d5a48ddCoriander4R_data.csv - 6,118 bytesMD5:
3d86ba5f9a5f3cad6260c945557656e5Coriander_4Rgermination.csv - 6,028 bytesMD5:
c89d63adf25f913bb7b149cd75b619e3Onion4R_data.csv - 5,809 bytesMD5:
9a700c693068835703c0eb365523c0c0Onion_4Rgermination.csv - 2,739 bytesMD5:
8e3b0925df458e2d838c8103a4cd68c0OnionPhoma_4Rdata.csv - 5,686 bytesMD5:
a9334b800d82e186b7bbe7bc81240a77OnionPhoma_SpinachFusarium.csv - 1,072 bytesMD5:
15f2508dc4777adb7e232bf3133e0f4dPepper_4Rgermination.csv - 23,121 bytesMD5:
d471c684fa56d434eb05572e1a8ce9ffPepper_plant_4Rdata.csv - 2,328 bytesMD5:
4c411521f046c8d5b38233da8425b085Pepper_seeds_4Rdata.csv - 4,845 bytesMD5:
b81b159551305b17010c5b5221205799Perennial_puccinia_4Rdata.csv - 4,845 bytesMD5:
01b8b47cc7bb9092c11207513a348d3bPerennial_thread_4Rdata.csv - 4,845 bytesMD5:
c653f6175bdd45dbcdac6093c4fd64eeRed_fescue_4Rdata.csv - 6,457 bytesMD5:
b631026f7bc3e7ca403a6276a7c73bf9Spinach4R_data.csv - 5,777 bytesMD5:
2a061fd09d20cf5e22386059f3ec9ca3Spinach_4Rgermination.csv - 2,599 bytesMD5:
87ec43a7a4f8ceb259e945e3697e2e82SpinachFusarium_4Rdata.csv - 1,802 bytesMD5:
cfa582163c92545ec91cc935da7f04e1summarySE_function.R -
download all files (zip)
296,943 bytes unzipped
