Data underlying the publication: Asymmetric mono-reduction of α,β-dicarbonyls to α-hydroxy carbonyls by ene reductases
This is a dataset that is used in the manuscript "Asymmetric mono-reduction of α,β-dicarbonyls to α-hydroxy carbonyls by ene reductases". Our aim in this manuscript is to show that OYE family of enzymes are able to catalyze the reduction of vicinal diketones. The dataset includes Nuclear Magnetic Resonance (NMR) Spectrometer results in MestReNova files, where each file is labelled as its reference from the manuscript figure. We also provide Gas Chromatography (GC) and High Pressure Liquid Chromatography (HPLC) data in the form of excel where chromatograms and the area and retention times have been copy/pasted. Along side those data are the calculations used to formulate the tables or figures in the manuscript, and are labelled according to their tables and figures as shown in the manuscript.
- 2024-09-26 first online, published, posted
- European Union's Horizon 2020 research and innovation programme (grant code 949910) European Research Council
- MSCA (grant code 101062327) European Union
DATA
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README.txt - 13,178,502 bytesMD5:
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Figure 3_various GC and HPLC results.xlsx - 1,264,265 bytesMD5:
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Figure 4_ 7a ERED screening results.xlsx - 452,467 bytesMD5:
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SI_figure S13_ BsGDH.xlsx - 4,774,761 bytesMD5:
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SI_figure S14_GC results 7a.xlsx - 198,493 bytesMD5:
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SI_figure S15 data.xlsx - 715,363 bytesMD5:
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SI_figure S26_bottom_OYE3_7a_BNAH.mnova - 246,242 bytesMD5:
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SI_figure S26_top_OYE3_7a_BNAH.mnova - 717,567 bytesMD5:
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SI_Figure S27 bottom_OYE3_7a_ddBNAH.mnova - 244,610 bytesMD5:
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SI_figure S27 top_ OYE3_7a_ddBNAH.mnova - 721,569 bytesMD5:
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SI_figure S28_top_OYE3_7a_BNAH_D2O.mnova - 352,281 bytesMD5:
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SI_figure S29_bottom_1_CARBON_20230529_01.mnova - 328,588 bytesMD5:
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SI_figure S33_Sample_5_prod_oye3_d2O_no_bnah_PROTON_20230531_01.mnova - 524,340 bytesMD5:
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SI_Figure S34_top_7b commercial standard.mnova - 359,114 bytesMD5:
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SI_Figure S35_bottom_commercial standard 7b.mnova - 326,716 bytesMD5:
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SI_Figure S37_purified_7b_PROTON_20240911_01.mnova - 228,029 bytesMD5:
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SI_Figure S41_phenylglyoxal.mnova - 222,487 bytesMD5:
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SI_Figure S42_2-hydorxyacetophenone.mnova - 223,346 bytesMD5:
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SI_Figure S50_trifluoro_44mM_PROTON_20240917_01.mnova - 273,559 bytesMD5:
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SI_Figure S51_trifluoro_standard_PROTON_20240917_01.mnova - 573,695 bytesMD5:
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Table 1_ cofactors and 7a.xlsx - 738,360 bytesMD5:
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Table 2_ extra substrates .xlsx -
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