The doi above is for this specific version of this dataset, which is currently the latest. Newer versions may be published in the future. For a link that will always point to the latest version, please use
doi: 10.4121/311fa522-2ce5-47f6-8674-10e001f7e909

Droste, Isabel; Schuitema, Erik; Khan, Sajjad; Heldens, Stijn; van Werkhoven, Ben et. al. (2024): Test data for Calibration-free estimation of field dependent aberrations for single molecule localization microscopy across large fields of view. Version 1. 4TU.ResearchData. dataset. https://doi.org/10.4121/311fa522-2ce5-47f6-8674-10e001f7e909.v1

Dataset

• Optical Physics
• Physical Sciences

microtubules, Single molecule localization microscopy, test data

CC BY 4.0

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by Isabel Droste , Erik Schuitema , Sajjad Khan , Stijn Heldens , Ben van Werkhoven , Keith A. Lidke , Sjoerd Stallinga , Bernd Rieger

Test data for the software demo of the manuscript 'Calibration-free estimation of field dependent aberrations for single molecule localization microscopy across large fields of view'. This dataset contains the first 800 frames from the 3D microtuble dataset from Figure 2 of the manuscript. It is a test dataset for the Software Demo accompanying the manuscript.

It concerns raw data for Single Molecule Localization Microscopy of microtubules of HeLa cells over a large 97 x 97 μm field of view. The images were acquired with 2 cylindrical lenses at the Fourier plane of the microscope to introduce astigmatism. The data was acquired in order to demonstrate the estimation of field dependent aberrations from single molecule localization.

• 2024-12-03 first online, published, posted

4TU.ResearchData

.tif

TU Delft, Faculty of Applied Sciences, Department of Imaging Physics