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Data underlying the publication: Phytophthora pathogens exploit slicing action for host invasion

dataset
posted on 22.03.2021, 14:40 by Jochem Bronkhorst, Joris Sprakel

Host entry requires plant pathogens to breach the protective surface of the plant. Phytophthora species, classified as oomycetes, are among the most destructive filamentous plant pathogens worldwide and pose a substantial threat to food security. Little is known about the biomechanics of host invasion in oomycetes, yet this understanding is crucial to combat these pathogens more effectively. Here we unveil how Phytophthora spp. invade elastic surfaces by pressure application at an oblique angle without appressorium formation. This ‘slicing’ action, coined the naifu-mechanism, facilitates crack initiation by concentrating stresses towards the surface enabling host entry through the crack void. This contrasts the ‘brute’ force approach found in fungal filamentous plant pathogens capable of producing a melanized appressorium that generates tremendous turgor pressures. Measurements of surface deformations during invasion reveal an asymmetric geometry of decoupled adherence and indentation sites that is quantitatively described with a mathematical model. Moreover, we establish how polarized growth, surface adherence and turgor generation are each essential to accomplish host entry by this approach. The naifu-mechanism enables Phytophthora pathogens to penetrate their hosts without necessitating specialized pressure organs and vast turgor pressures.

History

Publisher

4TU.ResearchData

Time coverage

2018-2021

Format

File Types: .nd2 files, raw format from a Nikon CLSM C2 confocal .tif files (large), lossless saving of .nd2 raw data for analysis. .tif files (small), exports of high resolution images for publication .eps/.png files, other figure exports used for publication .mat files, exports or contents of workspace of Matlab variables .fig files, figure exports of Matlab .m files, matlab code files (also found on github including explanation, https://github.com/jorissprakel/Phytopthora_invasion) .blend file, blender session instances that form the basis of several figures .avi/.mp4/.mov, movies of cells growing/moving over time. .opj files, Origin plot files used to make plots for the manuscript. .pptx files, generally to indicate which emasurements were done where. .txt, generally for notes on the research and details.

Organizations

Physical Chemistry and Soft Matter, Wageningen University and Research

Licence

Exports