Supplementary data for the paper: Simultaneous orientation and 3D localization microscopy with a Vortex point spread function
doi: 10.4121/13031837.v1
The doi above is for this specific version of this dataset, which is currently the latest. Newer versions may be published in the future.
For a link that will always point to the latest version, please use
doi: 10.4121/13031837
doi: 10.4121/13031837
Datacite citation style:
Hulleman, Christiaan; Thorsen, Rasmus; Stallinga, S. (Sjoerd); Rieger, B. (Bernd) (2020): Supplementary data for the paper: Simultaneous orientation and 3D localization microscopy with a Vortex point spread function. Version 1. 4TU.ResearchData. dataset. https://doi.org/10.4121/13031837.v1
Other citation styles (APA, Harvard, MLA, Vancouver, Chicago, IEEE) available at Datacite
Dataset
This dataset contains data collected as part of the development of the Vortex PSF. It consists of raw data to calibrate the aberrations over the field-of-view, verify the vortex PSF model, visualise transitions in orientation and perform Binding-Activated localization microscopy and orientation estimation on lambda-DNA. A drift corrected and non drift corrected processed lambda-DNA dataset is also included to show the possible parameters that one can visualize over the whole field-of-view.
The dataset is being made public both to act as supplementary data for publications and in order for other researchers to use this data to verify and develop the method and code.
Any reuse of this data should cite the original associated publication.
history
- 2020-10-02 first online, published, posted
publisher
4TU.ResearchData
references
funding
- Optical nanoscopy at 1 nm resolution: far-field fluorescence control at cryogenic temperatures (grant code 648580) [more info...] European Research Council
- Visualizing local and global chromatin architecture, and gene expression in the individual cell by structural (SUSHI) and temporal (3D-SMRT) single molecule imaging (grant code U01EB021238) [more info...] National Institute of Biomedical Imaging and Bioengineering
organizations
TU Delft, Faculty of Applied Sciences, Department of Imaging Physics
DATA
files
- 4,152,994,676 bytes md5 stk_0001_2500_2_middle_tryrefocus.tif
- 4,152,998,636 bytes md5 stk_0009_2500_2_middle_tryrefocus.tif
- 4,152,998,636 bytes md5 stk_0008_2500_2_middle_tryrefocus.tif
- 4,152,998,636 bytes md5 stk_0007_2500_2_middle_tryrefocus.tif
- 4,152,998,636 bytes md5 stk_0006_2500_2_middle_tryrefocus.tif
- 4,152,994,876 bytes md5 stk_0005_2500_2_middle_tryrefocus.tif
- 4,152,994,676 bytes md5 stk_0004_2500_2_middle_tryrefocus.tif
- 4,152,994,676 bytes md5 stk_0003_2500_2_middle_tryrefocus.tif
- 4,152,994,676 bytes md5 stk_0002_2500_2_middle_tryrefocus.tif
- 4,152,998,636 bytes md5 stk_0010_2500_2_middle_tryrefocus.tif
- 251,507,056 bytes md5 2500_2_X1_10.hdf5
- 207 bytes md5 2500_2_X1_10.yaml
- 230,548,388 bytes md5 2500_2_X1_10_drift_corrected.hdf5
- 356 bytes md5 2500_2_X1_10_drift_corrected.yaml
- 193,323,516 bytes md5 atto565_zstack_20perc_600ms_NOvortex_YESqwp_3.tif
- 193,323,516 bytes md5 atto565_zstack_20perc_600ms_YESvortex_YESqwp_3.tif
- 428,670,908 bytes md5 oil_3perc_300ms_100nmsteps_EPI_1.tif
- 428,670,908 bytes md5 oil_3perc_300ms_100nmsteps_EPI_2.tif
- 428,670,908 bytes md5 oil_3perc_300ms_100nmsteps_EPI_3.tif
- 428,670,908 bytes md5 oil_3perc_300ms_100nmsteps_EPI_4.tif
- 428,670,908 bytes md5 oil_3perc_300ms_100nmsteps_EPI_5.tif
- 428,670,908 bytes md5 oil_3perc_300ms_100nmsteps_EPI_6.tif
- 428,670,908 bytes md5 oil_3perc_300ms_100nmsteps_EPI_7.tif
- 428,670,908 bytes md5 oil_3perc_300ms_100nmsteps_EPI_8.tif
- 193,323,516 bytes md5 reorientation_ATTO_565_tirf_qwp_vortex_900ms.tif
- 4,477 bytes md5 README.txt
- download all files (zip)