Supplementary data for the paper: Simultaneous orientation and 3D localization microscopy with a Vortex point spread function

DOI:10.4121/13031837.v1
The DOI displayed above is for this specific version of this dataset, which is currently the latest. Newer versions may be published in the future. For a link that will always point to the latest version, please use
DOI: 10.4121/13031837

Datacite citation style

Hulleman, Christiaan; Thorsen, Rasmus; Stallinga, S. (Sjoerd); Rieger, B. (Bernd) (2020): Supplementary data for the paper: Simultaneous orientation and 3D localization microscopy with a Vortex point spread function. Version 1. 4TU.ResearchData. dataset. https://doi.org/10.4121/13031837.v1
Other citation styles (APA, Harvard, MLA, Vancouver, Chicago, IEEE) available at Datacite

Dataset

This dataset contains data collected as part of the development of the Vortex PSF. It consists of raw data to calibrate the aberrations over the field-of-view, verify the vortex PSF model, visualise transitions in orientation and perform Binding-Activated localization microscopy and orientation estimation on lambda-DNA. A drift corrected and non drift corrected processed lambda-DNA dataset is also included to show the possible parameters that one can visualize over the whole field-of-view.
The dataset is being made public both to act as supplementary data for publications and in order for other researchers to use this data to verify and develop the method and code.
Any reuse of this data should cite the original associated publication.

History

  • 2020-10-02 first online, published, posted

Publisher

4TU.ResearchData

Funding

  • Optical nanoscopy at 1 nm resolution: far-field fluorescence control at cryogenic temperatures (grant code 648580) [more info...] European Research Council
  • Visualizing local and global chromatin architecture, and gene expression in the individual cell by structural (SUSHI) and temporal (3D-SMRT) single molecule imaging (grant code U01EB021238) [more info...] National Institute of Biomedical Imaging and Bioengineering

Organizations

TU Delft, Faculty of Applied Sciences, Department of Imaging Physics

DATA

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