Dataset underlying the publication 'Optimization of Capture ELISAs for Chicken Cytokines Using Commercially Available Antibodies''
doi:10.4121/21494520.v1
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doi: 10.4121/21494520
doi: 10.4121/21494520
Datacite citation style:
Krzysica, Paulina; Verhoog, Loes; de Vries, Sonja; Smits, Coen; Savelkoul, H.F.J. (Huub) et. al. (2022): Dataset underlying the publication 'Optimization of Capture ELISAs for Chicken Cytokines Using Commercially Available Antibodies''. Version 1. 4TU.ResearchData. dataset. https://doi.org/10.4121/21494520.v1
Other citation styles (APA, Harvard, MLA, Vancouver, Chicago, IEEE) available at Datacite
Dataset
The research is about optimization and validation of capture ELISA for chicken IL-2, IL-6, IL-10, IL-12p40, and IFN-y using commercially available antibodies and recombinant cytokines.
To optimize antibody concentrations, checkerboard titration was performed. Then, validation tests for capture ELISAs were performed to assess lower limit of detection (LLOD), matrix effect, parallelism, and inter-assay variation.
history
- 2022-12-09 first online, published, posted
publisher
4TU.ResearchData
format
xlsx
associated peer-reviewed publication
Optimization of Capture ELISAs for Chicken Cytokines Using Commercially Available Antibodies
organizations
Cell Biology and Immunology, Wageningen University & ResearchAnimal Nutrition, Wageningen University & Research
DATA
files (10)
- 4,156 bytesMD5:
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README.txt - 17,343 bytesMD5:
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Figure3_checkerboard_titration_IL-2.xlsx - 14,418 bytesMD5:
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Figure4_calibration_curves.xlsx - 15,236 bytesMD5:
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Figure5_matrix_effect_plasma.xlsx - 15,349 bytesMD5:
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Figure6_matrix_effect_serum.xlsx - 17,482 bytesMD5:
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Figure7andTable4_parallelism.xlsx - 18,597 bytesMD5:
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FigureS1_checkerboard_titration_IL-2_Tween20.xlsx - 19,726 bytesMD5:
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Table3_lower_limit_of_detection.xlsx - 21,183 bytesMD5:
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Table5_inter-assay_variation.xlsx - 19,651 bytesMD5:
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TableS1_lower_limit_of_detection_serum.xlsx -
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