Data underlying the publication: Ultrasound Transparent Neural Interfaces for Multimodal Interaction - In vivo experiments

DOI:10.4121/52ab5985-e45a-4f17-828e-ccb7257d8265.v1
The DOI displayed above is for this specific version of this dataset, which is currently the latest. Newer versions may be published in the future. For a link that will always point to the latest version, please use
DOI: 10.4121/52ab5985-e45a-4f17-828e-ccb7257d8265

Datacite citation style

Panskus, Raphael; Giagka, Vasiliki (2025): Data underlying the publication: Ultrasound Transparent Neural Interfaces for Multimodal Interaction - In vivo experiments. Version 1. 4TU.ResearchData. dataset. https://doi.org/10.4121/52ab5985-e45a-4f17-828e-ccb7257d8265.v1
Other citation styles (APA, Harvard, MLA, Vancouver, Chicago, IEEE) available at Datacite

Dataset

Data sets of in vivo experiments (FUSI data of awake mice)

Functional ultrasound imaging sequence of in vivo experiments

Similar to the phantom experiments, ultrasound images were acquired using a 18 MHz linear array (L22-14vX with 128 piezoelectric elements, 100 μm pitch, 8 mm elevation focus, Verasonics, USA) connected to a programmable ultrasound scanner (Vantage 256 High frequency configuration, Verasonics, USA). We transmitted a sequence of 12 angled plane waves(-10° to 10°), resulting in a 1 kHz framerate after compounding. Ensembles of 200 compounded frames were acquired every

0.2 s for a total period of 1150 s, resulting in an effective power Doppler rate of 5 Hz. During acquisition, RF data was real time delay-and-sum beamformed to complex IQ frames, which where saved for later offline Doppler processing.


fUS acquisition of in vivo experiments

Mice were habituated to head-fixation on a running wheel in a dark environment (0- 2 lux) by progressively increasing the restraining period from 10 up to 90 minutes over 7 days. After the habituation period, an experiment session consisting of 2 recordings was conducted on two consecutive days when the animal was 13 weeks old. Mice were facing a monitor (U2410, Dell, USA) ∼20 cm in front of them. The ultrasound probe is fixed to a micromanipulator enabling adjustments to target the superior colliculus (SC). Acoustic gel (Aquasonic MAT-00-28152 ,Parker Laboratories, USA) was applied on the cranial window for ultrasound coupling before placing the ultrasound probe 2 mm above the TPX. For the first recording, functional scans were acquired without the NEP placed on the cranial window. During the second recording, the NEP was placed on the cranial window. The next experiment session was done in a reverse sequence. A recording consisted of 10 min of baseline

recording and 10 stimuli, each containing a 15-second-long visual stimulation with an interval stimulus interval (ISI) of 30 s baseline monitor display (grey screen). The visual stimuli consisted of black and white full-field drifting gratings (50 degrees per second, 30- 32 lux) moving in one of 8 different directions (angle: 0, 45, 90, 135, 180, 225, 225, 270, 315; randomized sequence) for 1 s each. Again, a ParC-based sample was used as NEP ( 2.5 μm ParC, 300 nm Au with a 50 nm Ti adhesion layer,

and 1.5 μm ParC insulation)

History

  • 2025-09-23 first online, published, posted

Publisher

4TU.ResearchData

Format

mat

Funding

  • Dutch Brain Interface Initiative (DBI2) [more info...] Ministry of Education, Culture and Science (OCW)
  • Medical Delta Program Ultrafast Heart and Brain

Organizations

TU Delft, Faculty of Electrical Engineering, Mathematics and Computer Science, Department of Microelectronics, Section Bioelectronics

DATA

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