***Data underlying the research of Daphnia magna exposure to chlorpyrifos*** Authors: Q. Lang, S. Li, Institute of Pesticides and Environmental Toxicology, College of Agriculture and Biotechnology, Zhejiang University  (Hangzhou, China) Corresponding author: S. Li Contact Information: snli@zju.edu.cn Hepingguan Building, Huajiachi Campus, Zhejiang University No. 268, Kaixuan Road, Hangzhou 310029, Zhejiang Province, China ***General Introduction*** This dataset contains data collected from a 39-day experiment in which a set of macrophyte- dominated freshwater indoor systems were employed: doi:10.4121/uuid:804cbaa6-95ec-4c28-829f-87ba0344a554 The experiment was conducted in Institute of Pesticides and Environmental Toxicology, College of Agriculture and Biotechnology, Huajiachi Campus, Zhejiang UniversityThis research project was made possible by a grant from the China Scholarship Council (CSC). ***Purpose of the test campaign*** The purpose of the experiment was to quantify relationship between state of biomarker (inherent activity of ChE, apparent activity of ChE, immuno-reactive content of NAGase, and activity of NAGase) and that of population ***Test equipment*** Shimadzu GC-2010 Plus (equipped with a ECD) GEMINI XPS fluorescence microplate reader VERSA max microplate reader, etc. ***Description of the data in this data set*** Figure 3: Change in population of Daphnia magna in chlorpyrifos expoxure. Figure 6: Change in somatic ChE (inherent activity and apparent activity) in D. magna in chlorpyrifos exposure. Figure 9: Change in immuno-reactive content and activity of NAGase in media.