Column name	Explanation
#SampleID	"Sample ID"
Library	"sequence library (1-5, is A-E)"
BarcodeNumber	"barcode primer set, 1-70, per sequence library"
ContainerID	"container identifier"
Timepoint	"sampled time point: day 0, 5, 10, or 15"
Diet	"type of feed substrate: CF = chicken feed, CM = chicken manure, CS = camelina"
Density	"larval density, in number of neonate larvae (0, 50, 100, 200) per container added to the feed substrate on day 0"
Replicate	"replicate within a Diet x Density x Timepoint x Type combination (1-4)"
Spare	"spare samples, options: no (main analysis), yes (additional samples: adult flies, eggs, biofilms, duplicate samples), na (positive/negative controls)"
Type	"sample type: larvae, substrate, eggs, biofilm, adults, na (technical samples)"
Details	"optional remarks on sample"
Duplicate	"Options: no (main analysis), biological (DNA isolation replicate), technical (PCR replicate), na (positive/negative controls)"
Category	"Options: control (no-template control of PCR), sample (all other samples)"
DNA_reading	"DNA concentration of PCR product, in ng/ul"
Description	"Sample ID"
	
"na" or "NA" in a field stands for "not applicable".