00:00:02
Speaker 1: So how was it like the second week for you?
00:00:06
Speaker 2: Um.
00:00:07
Speaker 1: It was a bit less responsive. The change is that it could make were bigger in the first week. In the second week there was still some change, but it was just less of it, basically. So.
00:00:21
Speaker 2: I see. Is there anything specific you'd like to share?
00:00:24
Speaker 1: Well, just things like the light responsiveness seemed less so. The purple dye was less intense. Um, where they're either dying or at least they're aggregating, either aggregating or dying in the area of nutrients. Um, so there are still areas where they're surviving and maybe even accumulating, but there's less, um, the purple color is a bit less. And when we tried to put prisms on it, I think that would have worked better in the first week. You can see something, but it's not as strong a difference as what.
00:01:01
Speaker 2: Did you see?
00:01:02
Speaker 1: Well, um, you can see a little bit the outlines. I don't know whether it's from the pressure or because it's pushing the light somewhere else, but there is a little but it's not a not a big thing.
00:01:16
Speaker 2: You mean the. There's a little square here? Yes. Yeah, I also see it. Yeah, but the light coming from the prism. Did it influence the other area?
00:01:26
Speaker 1: It didn't seem to have a really it had some effect, I think, but it didn't have a really that big an effect. Okay.
00:01:36
Speaker 2: So you tried something new, like the prison?
00:01:39
Speaker 1: Yeah, I think it's just a variation on what we did before, but. Yes.
00:01:43
Speaker 2: Yeah. Yeah. What else did you try?
00:01:45
Speaker 1: Um, not that much. We tried moving it a few more times, as I say, because the changes were smaller, we didn't really see that much extra, basically. Okay.
00:01:57
Speaker 2: What would you say is the is its favorite place or spots?
00:02:02
Speaker 1: Um, I think on the whole there seems to work. It gets quite a lot of light or if you want it to be more active in the afternoon evenings putting it at that. But it's certainly in the light somewhere.
00:02:18
Speaker 2: Yeah. So you were constantly also changing according to the light.
00:02:22
Speaker 1: Um, not constantly, but sometimes, yes. Okay.
00:02:25
Speaker 2: Um, when did you notice the green turn? Returning back on the. On the upper part where it was kind of.
00:02:34
Speaker 1: Well, I noticed a few days ago that it had started to. I don't know. It might be. It's accumulating. I'm not sure. But I. I noticed that patterning a few days ago. Yeah.
00:02:47
Speaker 2: And why do you think it became like that?
00:02:50
Speaker 1: Well, I think there's a lot less bacteria and a lot less nutrients, but. They also seem to be accumulating. So they seem to be denser in some areas. Here they're spread, but here they're actually more intense. But in very and I think that's probably a survival mechanism. It's something to do with forming a biofilm or resisting stress or something like that.
00:03:14
Speaker 2: I see. Yeah. So how did you feel when you noticed the first of all, the change in the second week is less than the first week?
00:03:24
Speaker 1: Um, not really feeling that much. I mean, I was kind of expecting it. Okay, so, yeah.
00:03:31
Speaker 2: So you sort of knew.
00:03:33
Speaker 1: But I suspected it was going to perform less well as time went on because it was obvious from the way it was moving that it was using up the nutrients.
00:03:44
Speaker 2: I see.
00:03:45
Speaker 1: So it was not an infinitely sustainable system.
00:03:49
Speaker 2: So yeah, some nutrients needs to be added, apparently.
00:03:53
Speaker 1: Yes. I mean, I would have thought what nutrients do you add?
00:03:58
Speaker 2: The PG 11 and some trace metal?
00:04:01
Speaker 1: What's G 11?
00:04:02
Speaker 2: It's basically a standard medium for the cyanobacteria, right. Um, there are a couple of things in it stuff, chemicals in it, and then there are some also metal elements.
00:04:18
Speaker 1: Yeah. And I, I would imagine that the trace elements aren't being used up. The question is what else it has in it. So it might have a nitrogen source or possibly even a carbon source. Um, the other thing that made me wonder was the. I guess. The polymer that presumably the pdms. Is it also Co two permeable?
00:04:42
Speaker 2: Yes.
00:04:43
Speaker 1: Okay. So it can fix carbon from outside. Okay. Yeah. Okay.
00:04:47
Speaker 2: Yeah. That's why the biomass is accumulating. Yeah. Yeah. And do you notice there's a bit of bubble inside?
00:04:57
Speaker 1: Yes. That was towards the end of the first week. There seemed to be and I don't know what that is. I don't know whether it's gases coming from the organism. Um, maybe it is because this doesn't seem to do it, whereas this does it. So I guess not all gases can get out completely easily. And I suppose that might also explain a bit the change in behavior. I see.
00:05:23
Speaker 2: So there are other types of gases. Could be. There could.
00:05:27
Speaker 1: Be. There could be. I mean, I would imagine that they're they want oxygen. At night. And they and they want CO2 in the day. So there must be a sort of different cycle. But maybe they're producing signaling compounds or methane or other. I don't know much about cyanobacteria. So.
00:05:52
Speaker 2: Um. Okay. Talked about your feelings, and now we talk about the time you spent. Did you find yourself spending less time or more time on it for the second week?
00:06:07
Speaker 1: Less time.
00:06:08
Speaker 2: Less time? Yeah. Yeah. Why is that?
00:06:11
Speaker 1: Just because it wasn't doing as much. It was interesting. The first week I sent you a lot more pictures than the second week, and that's because more was happening. So. Yeah.
00:06:22
Speaker 2: And I assume it also didn't really change or cause impact on your own life or anything. Okay. Um, did you feel more motivated to look after it or less motivated?
00:06:37
Speaker 1: Um, I didn't think there was much I could do. If I had the nutrients, I would have tried something, but I didn't think there was. I think the only thing we could do is not damage it. Yeah, I didn't think there was much that we could add.
00:06:59
Speaker 2: You mean the kind of the. The light is there and everything is sort of what you can try. You try it? Yes. Actually, in the first week, I noticed you. You send a picture. You also bring it outside. Yes. You were having breakfast?
00:07:16
Speaker 1: Uh, I can't remember what time of day it was, but it might have been. Yes. Yeah.
00:07:21
Speaker 2: Why did you do that?
00:07:22
Speaker 1: Well, the thing that I'm not still not quite sure about is why it responds better to sunlight than. A white led light because white LEDs also are quite good with broad spectrum. They're not like fluorescent lights. So when we grow other organisms, we find white LEDs work better. But here it seemed to be sunlight that was working better. And I don't really know what bit of the spectrum was different or why or whether it was just intensity. But yeah.
00:07:56
Speaker 2: Yeah. So you were trying out. Yeah. Okay. Yeah. Because once you put a white led during the night, right?
00:08:03
Speaker 1: Yeah. But it made a little difference. But I didn't think it made much. Okay. And we put a red led on before that and I wasn't expecting the red led to do so much because it's obviously a narrower wavelength, but I thought the white would do more than the Led, but.
00:08:20
Speaker 2: They were sort of this.
00:08:22
Speaker 1: Yeah, they're similar. Okay. Yeah.
00:08:25
Speaker 2: So the organism is kind of. Mysterious still or doesn't really.
00:08:35
Speaker 1: No, not really. I mean, I just think. There's stuff to know. I don't find it mysterious, but that's because I work on something quite similar. Yeah.
00:08:47
Speaker 2: I do find your. You have a very strong, um, curiosity or experimentation spirit in trying out different things. Yeah, that's quite unique, actually. Okay. Okay. Do you consider them actually as care, though?
00:09:06
Speaker 1: Um. Could you say that again, please?
00:09:10
Speaker 2: So your experiments or your curiosity or your wanting to know more about how it works, basically, do you consider it also as care? Um.
00:09:24
Speaker 1: Not. Really? No. They don't have the function of a pet or anything like that. Um, yeah.
00:09:41
Speaker 2: Yeah. So for you, it's basically like an object to. To, to sort of. Yeah, that's interesting. Yes. Okay, great. So the function is quite important.
00:09:54
Speaker 1: Um. Yes, it is. Yes.
00:09:58
Speaker 2: Okay. Yeah, good to know. And, uh, last two questions. First, how much of a good carer do you think you were over the two weeks?
00:10:08
Speaker 1: Well, I don't think we tried hard to care for it, but also we tried reasonably hard not to damage it. Okay, so.
00:10:16
Speaker 2: Yeah, damage.
00:10:17
Speaker 1: Well, so we knew that if we really put it in hot sunlight for a long period it would be damaged. So we avoided doing anything like that.
00:10:26
Speaker 2: So you're trying kind of a viable situation where it can survive. But yes, you were not, um, say like, try your best to create a. Yeah. Yeah. Okay. Why did you why, why, why did you choose this path?
00:10:44
Speaker 1: Well, because, as I say, I couldn't think apart from illumination what to do that would make it perform better. So I just. I just couldn't see. If it had been a plant, we would have watered it or, you know, there are more things that we can do. Yeah. Yeah. But here, because it's a partially closed system. We don't have easy ways except for light of modifying the system.
00:11:12
Speaker 2: Yeah. Interesting. So you feel also quite limited in things you can do? Yeah. Yeah. Huh? Yeah. So if if let's imagine it's sort of more open system and you could add things. You could remove things. Yes. You would expect yourself doing. Doing more or caring more for it. Yes. Okay. Yeah. And in the end, yeah, we sort of already talked about it. But the question is, what do you think would help you to improve your care for the artifact? But that we talked about, uh, if it being a more open system.
00:11:54
Speaker 1: Yes.
00:11:56
Speaker 2: Um, what else would help you?
00:11:57
Speaker 1: Well, a more open system is one thing. A more effectively reproductive system is another thing. So if you gave us. Ten squares like that. Nine of them didn't have they had the nutrients, but they didn't have the cyanobacteria. One of them did. And you gave us a way to transfer from one to the other. So things like that. So basically, the more.
00:12:28
Speaker 2: Um, the more the.
00:12:29
Speaker 1: System can function by itself or we have ways of intervening to make it function better, the more interesting it is. Mm.
00:12:38
Speaker 2: I see. Yeah. So in the end, it boils down to the fact that the artifact being very, um, how do we, like, sustain itself? That's you can't really intervene to. Yes. To even make it better. Yes. Okay. Yeah. Nice. Good insights. Thank you. Thank you very much.



Colin 3.m4a



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