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GENERAL INFORMATION

This dataset is part of Chapter 2 of the PhD thesis "E(gg)xit strategy of plant defense: Evolution and genetics of a butterfly egg-triggered cell death"
doi: https://doi.org/10.18174/575127

Title of the dataset:
"Insect egg-killing: a new front on the evolutionary arms-race between brassicaceous plants and pierid butterflies"
doi: https://doi.org/10.18174/575127

Creator:
Name:	Niccolo Bassetti
Institution: Biosystematics Group (Wageningen University & Research)
ORCID: https://orcid.org/0000-0003-1859-4698
Email: niccolo.bassetti@protonmail.com

Abstract:
A novel front-line of plant defence is the killing of herbivorous insect eggs with an HR reponse. We test whether this HR-based egg-killing plant trait has an evolutionary basis in the plant–insect arms-race.  We studied the phylogenetic distribution of this trait, its egg-killing effect on and elicitation by butterflies, by screening 31 Brassicales species, and nine Pieridae species.

Keywords:
coevolution, counter adaptation, egg deposition, hypersensitive response, induced plant, defences, plant toxins, specialist herbivores

Date of data collection:
2017-2018

Funding: 
The research described in this thesis was financially supported by the Toegepaste en Technische Wetenschappen (TTW), which is part of the Nederlandse Organisatie voor Wetenschappelijk Onderzoek (NWO), through the NWO/TTW VIDI grant no. 14854 awarded to dr. Nina E. Fatouros (Biosystematics Group, WUR), and partly co-founded (<10%) by seed companies Syngenta and Hazera (Vilmorin & Cie group).


DATA & FILE OVERVIEW

File list:
Files are arranged in the following folders, with each folder referrring to a figure of the manuscript/article.

figure_1:	figure_1.xlsx - data on the elicitation of HR on Brassicaceae and Cleomaceae species (raw data and summary statistics)
figure_2:	figure_2.xlsx - data from qPCR-RT on PR1 gene expression induced by egg washes of different Lepidoptera species.
			qPCR.R - R script used for the data analysis and plotting of qPCR-RT data
			qPCR.txt - dataset used with the qPCR.R script
figure_3:	stat_Survbnr.R	- R script used for data analysis of P. brassicae survival data on B. nigra
			Survbnr.csv - dataset used with stat_Survbnr.R script
			stat_Survival.R - R script used for data analysis of P. brassicae survival data on B. napus and C. hispanica
			Survival.csv - dataset used with stat_Survival.R script 
			plot_Survival.R	- R script used for plotting of survival data
			Survival_Arabidopsis.txt - A. thaliana dataset used with plot_Survival.R script 
			Survival_Bnigra.txt - B. nigra dataset used with plot_Survival.R script
			Survival_Brassica.txt - dataset used with plot_Survival.R script
figure_S2:	script_figure_S2.R	- R script used for data analysis and plotting of plant images with HR necrosis and histochemical stainings (ROS and TB)
			figure_S2.txt - dataset used with script_figure_S2.R script


METHODOLOGICAL INFORMATION

Description of methods used for collection/generation of data:
figure_1:	plant and butterfly phylogentic relationships were inferred using DNA sequences of nuclear (and/or) chloroplast markers
			1a) presence/absence of HR induced by P. brassicae eggs on plants was assessed through a biossay. Plants were treated with either egg wash or control water wash and visually scoring HR severity. 
			1b) ability of Pieridae eggs to induce HR on B. nigra plants was assessed through a biossay. Plants were treated with egg wash of different butterflies/moths and visually scoring HR severity
figure_2:	2a) fraction of B. nigra plants showing a HR response was assessed with same bioassay as in figure 1. 
			2b) relative expression of PR1 gene expression was measured through qPCR-RT.
figure_3:	P. brassicae egg survival was assessed by forcing butterflies to lay single eggs on leaves. egg survival/mortality was assessed six days after oviposition by visually counting hatched eggs compare to total laid eggs.
figure_4:	Phylogenetic relationships are according to Wiemers et al. (2020), and oviposition traits and host plants were retrieved from Tolman (2001).
figure_S1:	Presence abscence of HR on B. nigra leaves was assessed through a bioassay same as in figure 1 and 2. 
figure_S2:	eggs were laid on plants through a bioassay. ROS and cell death were visualized through histochemical stainings. staining amount was quantified through image analysis.

		
Methods, materials and software for processing the data:
Available in the Material and Methods section of the manuscript
doi: https://doi.org/10.18174/575127


SHARING/ACCESS INFORMATION

This dataset is published under the CC BY (Attribution) license.
This license allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator.


