README for Figure S2A.csv 
*** This file contains the raw data obtained on DNA and DNA-HMfA K31A E35A complexes using microscale thermophoresis experiments represented in Figure S2A of 
Article: Specific DNA binding of archaeal histones HMfA and HMfB
Authors: Erkelens, Henneman, van der Valk, Kirolos and Dame
Journal:  
DOI:
Corresponding author: rtdame@chem.leidenuniv.nl 


Legend Figure S2:Binding of HMf derivatives to Nonspecific and Clone20 DNA substrates. Normalized thermophoresis 
curves of Nonspecifc or Clone20 DNA as a function of A) HMfAK31A E35A or B) HMfBD14A K30A E34A. For HMfB WT, an 
extra curve on Nonspecific DNA was measured with 0.2% Tween20 in the buffer. Error bars indicate the standard 
deviation of three independent measurements. Dashed lines are lines to guide the eye. Wildtype curves from WT 
proteins (figure 3) are included in dark (Nonspecific) and light (Clone20) grey for easy comparison.

*** The data were obtained using microscale thermophoresis experiments as described in the associated article. 

***The data are labeled by type of DNA used (Nonspecific/Clone20) as represented in figure S2A.
Column A: Amount of HMfA K31A E35A in micromolar
Column B: Fnorm values replicate 1
Column C: Fnorm values replicate 2
Column D: Fnorm values replicate 3
Column E: Fnorm values replicate 4










