README for Figure S4A Clone20R.csv 
*** This file contains the raw data obtained on DNA and DNA-HMfA complexes using tethered particle motion represented in Figure S4A of 
Article: Specific DNA binding of archaeal histones HMfA and HMfB
Authors: Erkelens, Henneman, van der Valk, Kirolos and Dame
Journal:  
DOI:
Corresponding author: rtdame@chem.leidenuniv.nl;

Legend Figure S4:Binding of HMfA and HMfB to Clone20L or Clone20R in TPM experiments Root mean square displacement 
(RMS) of Clone20L and Clone20R DNA incubated with A) HMfA or B) HMfB in 50 mM Tris-HCl pH 7, 75 mM KCl. 
Histograms were fitted to a Gaussian distribution. Error bars represent the propagated standard deviation of 
two replicates. Dashed lines are lines to guide the eye. C) Binding curve of HMfB on Clone20R DNA. Data point 
were fitted using the Hill binding model. D) Calculated end-to-end distance for bound and unbound Clone20R DNA 
incubated with 30 nM HMfB. Histograms were fitted with a skewed normal distribution. Insert: pairwise 
distribution plot of the difference between the two populations. Histogram was fitted with a Gaussian 
distribution. 

 *** The data were obtained using Tethered particle motion as described in the associated article. 

*** Data obtained for each protein concentration(s) is given in this table as plotted in figure S4A.
Column A: HMfA (nM) = Concentration of HMfA in nanomolar
Column B: Replicate = number of replicate
Column C: RMS (nm) = Root mean square deviation in nanometer measured for each bead 
Column D: rho = anisotropic ratio (movement in x-direction/movement in y-direction) 
Column E: stdev = standard deviation 
Column F: FoV = field of view (data with the same FoV were collected in one image) 
Column G: bead = beads in each FoV are represented by a unique number 
The values given in column D and E are tether quality criteria and used for data filtering as described in the associated article.