README for Figure S1 insert.csv 
*** This file contains the raw data obtained on DNA and DNA-HMfB complexes using tethered particle motion represented in Figure S1 insert of 
Article: Specific DNA binding of archaeal histones HMfA and HMfB
Authors: Erkelens, Henneman, van der Valk, Kirolos and Dame
Journal:  
DOI:
Corresponding author: rtdame@chem.leidenuniv.nl;

Legend Figure S1: Calculated end-to-end distances for the unbound and bound population of 21 nM HMfB on Clone20 
DNA. Histograms were fitted with a skewed normal distribution, resulting in end-to-end distances of 102.2 ± 10.4 
nm and 78.64 ± 10.8 nm for unbound and bound DNA respectively. Insert: pairwise distribution plot of the 
differences between the two end-to-end distance peaks. Histogram was fitted with a Gaussian distribution 
resulting in a difference of 22.95 ± 9.3 nm. 

 *** The data were obtained using Tethered particle motion as described in the associated article. 

*** Data obtained for 21 nM HMfB is given in this table as plotted in figure S1 insert.
Column A: peak_unbound_sample = end-to-end distance used from replicate_FoV_bead from unbound peak as in S1
Column B: peak_bound_nm_sample = end-to-end distance used from replicate_FoV_bead from bound peak as in S1
Column C: peak_unbound_value = end-to-end distance (nm) for bead indicated in column A
Column D: peak_bound_value = end-to-end distance (nm) for bead indicated in column B
Column E: peak_unbound_minus_bound = difference between column E and D (nm)