README for Figure S4D insert.csv 
*** This file contains the raw data obtained on DNA and DNA-HMfB complexes using tethered particle motion represented in Figure S4D insert of 
Article: Specific DNA binding of archaeal histones HMfA and HMfB
Authors: Erkelens, Henneman, van der Valk, Kirolos and Dame
Journal:  
DOI:
Corresponding author: rtdame@chem.leidenuniv.nl;

Legend Figure S4:Binding of HMfA and HMfB to Clone20L or Clone20R in TPM experiments Root mean square 
displacement (RMS) of Clone20L and Clone20R DNA incubated with A) HMfA or B) HMfB in 50 mM Tris-HCl pH 7, 
75 mM KCl. Histograms were fitted to a Gaussian distribution. Error bars represent the propagated standard 
deviation of two replicates. Dashed lines are lines to guide the eye. C) Binding curve of HMfB on Clone20R 
DNA. Data point were fitted using the Hill binding model. D) Calculated end-to-end distance for bound and 
unbound Clone20R DNA incubated with 30 nM HMfB. Histograms were fitted with a skewed normal distribution. 
Insert: pairwise distribution plot of the difference between the two populations. Histogram was fitted with 
a Gaussian distribution. 

 *** The data were obtained using Tethered particle motion as described in the associated article. 

*** Data obtained for 30 nM HMfB is given in this table as plotted in figure S4D insert.
Column A: peak_unbound_sample = end-to-end distance used from replicate_FoV_bead from unbound peak as in S4D
Column B: peak_bound_nm_sample = end-to-end distance used from replicate_FoV_bead from bound peak as in S4D
Column C: peak_unbound_value = end-to-end distance (nm) for bead indicated in column A
Column D: peak_bound_value = end-to-end distance (nm) for bead indicated in column B
Column E: peak_unbound_minus_bound = difference between column E and D (nm)