README for Figure 1D.csv 
*** This file contains the raw data obtained on DNA and DNA-HMfA complexes using tethered particle motion represented in Figure 1D of 
Article: Specific DNA binding of archaeal histones HMfA and HMfB
Authors: Erkelens, Henneman, van der Valk, Kirolos and Dame
Journal:  
DOI:
Corresponding author: rtdame@chem.leidenuniv.nl;

Legend Figure 1:HMfA and HMfB bind as tetramers to the Clone20 site preceeding hypernucleosome formation A) 
Root mean square displacement (RMS) values of Nonspecific and Clone20 DNA tethers incubated with HMfA and B) 
with HMfB measured by TPM in 50 mM Tris-HCl pH 7, 75 mM KCl. Histograms were fitted with a Gaussian function 
and the mean values are represented by red and blue dots, respectively. Data for Nonspecific DNA was reproduced 
from Henneman et al. (12) and depicted as a line to guide the eye. Error bars represent the propagated standard 
deviation of at least two replicates C) Binding curve for specific binding of HMfB to the Clone20 substrate. 
The data points were fitted using the Hill binding model. Error bars represent the standard deviation of the 
replicates and propagated error for data points at saturation. D) Calculated end-to-end distances for unbound 
Clone20 DNA and with 5 nM HMfA. Histograms were fitted with a skewed normal distribution. Insert: pairwise 
distribution plot of the difference between the two end-to-end distance populations. Histogram was fitted with 
a Gaussian distribution.

 *** The data were obtained using Tethered particle motion as described in the associated article. 


*** Data obtained for 0 and 5 nM HMfA is given in this table as plotted in figure 1D.
Column A: HMfA (nM) = concentration of HMfA in nanomolar
Column B: Replicate = number of replicate
Column C: FoV = field of view (data with the same FoV were collected in one image) 
Column D: bead = beads in each FoV are represented by a unique number
Column E: 2.5% highest d (nm) = points with highest distance from center of the bead
Column F: end-to-end distance (nm) = distance calculated using d and triangular calculations
The values given in column A-D indicate which bead was selected and E and F are calculated as described in the associated article.