README for Figure 2B.csv 
*** This file contains the raw data obtained on DNA and DNA-HMfB D14A K30A E34A complexes using tethered particle motion represented in Figure 2B of 
Article: Specific DNA binding of archaeal histones HMfA and HMfB
Authors: Erkelens, Henneman, van der Valk, Kirolos and Dame
Journal:  
DOI:
Corresponding author: rtdame@chem.leidenuniv.nl;

Legend Figure 2:Histone derivatives HMfA K31A E35A and HMfB D14A K30A E34A recognize the Clone20 sequence A) Root
mean square displacement (RMS) values of Clone20 DNA tethers with HMfA K31A E35A or B) HMfB D14A K30A E34A as 
measured by TPM in 50 mM Tris-HCl pH 7, 75 mM KCl. Wildtype data was reproduced from figure 1. Histograms were 
fitted to a Gaussian distribution. Error bars represent the propagated standard deviation of at least two 
replicates. Dashed lines are to guide the eye.

 *** The data were obtained using Tethered particle motion as described in the associated article. 

*** Data obtained for each protein concentration(s) is given in this table as plotted in figure 2B.
Column A: HMfB D14A K30A E34A (nM) = Concentration of HMfB D14A K30A E34A in nanomolar
Column B: Replicate = number of replicate
Column C: RMS (nm) = Root mean square deviation in nanometer measured for each bead 
Column D: rho = anisotropic ratio (movement in x-direction/movement in y-direction) 
Column E: stdev = standard deviation 
Column F: FoV = field of view (data with the same FoV were collected in one image) 
Column G: bead = beads in each FoV are represented by a unique number 
The values given in column D and E are tether quality criteria and used for data filtering as described in the associated article.